PMID- 22505814
OWN - NLM
STAT- MEDLINE
DCOM- 20120907
LR  - 20220321
IS  - 1110-7251 (Electronic)
IS  - 1110-7243 (Print)
IS  - 1110-7243 (Linking)
VI  - 2012
DP  - 2012
TI  - Physical properties and biocompatibility of a core-sheath structure composite 
      scaffold for bone tissue engineering in vitro.
PG  - 579141
LID - 10.1155/2012/579141 [doi]
LID - 579141
AB  - Scaffolds play a critical role in the practical realization of bone tissue 
      engineering. The purpose of this study was to assess whether a core-sheath 
      structure composite scaffold possesses admirable physical properties and 
      biocompatibility in vitro. A novel scaffold composed of poly(lactic-co-glycolic 
      acid)/beta-tricalcium phosphate (PLGA/beta-TCP) skeleton wrapped with Type I collagen 
      via low-temperature deposition manufacturing (LDM) was prepared, and bone 
      mesenchymal stem cells (BMSCs) were used to evaluate cell behavior on the 
      scaffold. PLGA/beta-TCP skeleton was chosen as the control group. Physical 
      properties were evaluated by pority ratio, compressive strength, and Young's 
      modulus. Scanning electron microscope (SEM) was used to study morphology of 
      cells. Hydrophilicity was evaluated by water absorption ratio. Cell proliferation 
      was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay 
      (MTT). Osteogenic differentiation of BMSCs was evaluated by alkaline phosphates 
      activity (ALP). The results indicated that physical properties of the novel 
      scaffold were as good as those of the control group, hydrophilicity was 
      observably better (P < 0.01) than that of control group, and abilities of 
      proliferation and osteogenic differentiation of BMSCs on novel scaffold were 
      significantly greater (P < 0.05) than those of control group, which suggests that 
      the novel scaffold possesses preferable characteristics and have high value in 
      bone tissue engineering.
FAU - Wang, Chuangjian
AU  - Wang C
AD  - Institute of Orthopaedics and Traumatology, Xijing Hospital, The Fourth Military 
      Medical University, Xi'an 710032, China. wangchuang20051983@126.com
FAU - Meng, Guolin
AU  - Meng G
FAU - Zhang, Laquan
AU  - Zhang L
FAU - Xiong, Zuo
AU  - Xiong Z
FAU - Liu, Jian
AU  - Liu J
LA  - eng
PT  - Journal Article
DEP - 20120315
PL  - United States
TA  - J Biomed Biotechnol
JT  - Journal of biomedicine & biotechnology
JID - 101135740
RN  - 0 (Biocompatible Materials)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Collagen Type I)
RN  - 0 (beta-tricalcium phosphate)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/*chemistry/*pharmacology
MH  - Calcium Phosphates/chemistry/pharmacology
MH  - Cell Physiological Phenomena/drug effects
MH  - Cells, Cultured
MH  - Collagen Type I/chemistry/pharmacology
MH  - Femur/cytology
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Lactic Acid/chemistry/pharmacology
MH  - Materials Testing/methods
MH  - Mesenchymal Stem Cells/cytology/drug effects/metabolism
MH  - Osteoblasts/cytology/drug effects
MH  - Osteogenesis/*drug effects
MH  - Polyglycolic Acid/chemistry/pharmacology
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Porosity
MH  - Rabbits
MH  - Tibia/cytology
MH  - Tissue Engineering/*instrumentation
MH  - Tissue Scaffolds/*chemistry
PMC - PMC3312380
EDAT- 2012/04/17 06:00
MHDA- 2012/09/08 06:00
PMCR- 2012/03/15
CRDT- 2012/04/17 06:00
PHST- 2011/11/01 00:00 [received]
PHST- 2011/12/17 00:00 [revised]
PHST- 2012/01/04 00:00 [accepted]
PHST- 2012/04/17 06:00 [entrez]
PHST- 2012/04/17 06:00 [pubmed]
PHST- 2012/09/08 06:00 [medline]
PHST- 2012/03/15 00:00 [pmc-release]
AID - 10.1155/2012/579141 [doi]
PST - ppublish
SO  - J Biomed Biotechnol. 2012;2012:579141. doi: 10.1155/2012/579141. Epub 2012 Mar 
      15.