PMID- 22507241
OWN - NLM
STAT- MEDLINE
DCOM- 20131101
LR  - 20131121
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 21
IP  - 11
DP  - 2012
TI  - Granulocyte colony-stimulating factor and interleukin-1beta are important cytokines 
      in repair of the cirrhotic liver after bone marrow cell infusion: comparison of 
      humans and model mice.
PG  - 2363-75
LID - 10.3727/096368912X638856 [doi]
AB  - We previously described the effectiveness of autologous bone marrow cell infusion 
      (ABMi) therapy for patients with liver cirrhosis (LC). We analyzed chronological 
      changes in 19 serum cytokines as well as levels of specific cytokines in patients 
      after ABMi therapy and in a mouse model of cirrhosis generated using green 
      fluorescent protein (GFP)/carbon tetrachloride (CCl4). We measured expression 
      profiles of cytokines in serum samples collected from 13 patients before and at 1 
      day and 1 week after ABMi. Child-Pugh scores significantly improved in all of 
      these patients. To analyze the meaning of early cytokine change, we infused 
      GFP-positive bone marrow cells (BMCs) into mice with CCl4-induced LC and obtained 
      serum and tissue samples at 1 day and as well as at 1, 2, 3, and 4 weeks later. 
      We compared chronological changes in serum cytokine expression in humans and in 
      the model mice at 1 day and 1 week after BMC infusion. Among 19 cytokine, both 
      granulocyte colony-stimulating factor (G-CSF) and interleukin-1beta(IL-1beta) in serum 
      was found to show the same chronological change pattern between human and mice 
      model. Next, we examined changes in cytokine expression in cirrhosis liver before 
      and at 1, 2, 3, and 4 weeks after BMC infusion. Both G-CSF and IL-1beta were 
      undetectable in the liver tissues before and at 1 week after BMC infusion but 
      increased at 2 weeks and continued until 4 weeks after infusion. The infused BMCs 
      induced an early decrease of both G-CSF and IL-1beta in serum and an increase in the 
      model mice with LC. These dynamic cytokine changes might be important to repair 
      liver cirrhosis after BMC infusion.
FAU - Mizunaga, Yuko
AU  - Mizunaga Y
AD  - Department of Gastroenterology and Hepatology, Yamaguchi University Graduate 
      School of Medicine, Yamaguchi, Japan.
FAU - Terai, Shuji
AU  - Terai S
FAU - Yamamoto, Naoki
AU  - Yamamoto N
FAU - Uchida, Koichi
AU  - Uchida K
FAU - Yamasaki, Takahiro
AU  - Yamasaki T
FAU - Nishina, Hiroshi
AU  - Nishina H
FAU - Fujita, Yusuke
AU  - Fujita Y
FAU - Shinoda, Koh
AU  - Shinoda K
FAU - Hamamoto, Yoshihiko
AU  - Hamamoto Y
FAU - Sakaida, Isao
AU  - Sakaida I
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120410
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1beta)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Bone Marrow Transplantation/methods
MH  - Cell- and Tissue-Based Therapy/methods
MH  - Cytokines/*blood
MH  - Granulocyte Colony-Stimulating Factor/*blood
MH  - Humans
MH  - Interleukin-1beta/*blood
MH  - Liver Cirrhosis/*blood/*therapy
MH  - Liver Regeneration/physiology/radiation effects
MH  - Mice
MH  - Middle Aged
MH  - Young Adult
EDAT- 2012/04/18 06:00
MHDA- 2013/11/02 06:00
CRDT- 2012/04/18 06:00
PHST- 2012/04/18 06:00 [entrez]
PHST- 2012/04/18 06:00 [pubmed]
PHST- 2013/11/02 06:00 [medline]
AID - ct0254mizunaga [pii]
AID - 10.3727/096368912X638856 [doi]
PST - ppublish
SO  - Cell Transplant. 2012;21(11):2363-75. doi: 10.3727/096368912X638856. Epub 2012 
      Apr 10.