PMID- 22508056
OWN - NLM
STAT- MEDLINE
DCOM- 20120809
LR  - 20220318
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Print)
IS  - 1015-8987 (Linking)
VI  - 29
IP  - 3-4
DP  - 2012
TI  - mTOR-dependent modulation of gastric nesfatin-1/NUCB2.
PG  - 493-500
LID - 10.1159/000338503 [doi]
AB  - BACKGROUND: Nesfatin-1, an 82 amino acid peptide derived from the prohormone 
      nucleobindin-2 (NUCB2), is a novel satiety hormone acting through a 
      leptin-independent mechanism in the hypothalamus. The mechanisms by which 
      production of nesfatin-1/NUCB2 is regulated remain unknown. METHODS: 
      Nesfatin-1/NUCB2 mRNA and immunoreactivity were examined in gastric tissue and 
      Min-6 cells by RT-PCR and immunofluorescent staining or Western blotting. 
      RESULTS: Nesfatin-1/NUCB2 is co-localized with pS6K1, the downstream target of 
      mammalian target of rapamycin (mTOR), in gastric X/A like cells. A parallel 
      relationship between gastric mTOR signaling and nesfatin-1/NUCB2 was observed 
      during changes in energy status. Both mTOR activity and gastric nesfatin-1/NUCB2 
      were down-regulated by fasting, and returned to basal levels with re-feeding. In 
      high fat diet induced obese mice, gastric mTOR signaling and nesfatin-1/NUCB2 
      were increased. Inhibition of the gastric mTOR signaling by rapamycin attenuated 
      the expression of gastric nesfatin-1/NUCB2 mRNA and protein in both lean and 
      obese mice. Attenuation of mTOR activity by rapamycin or over-expression of TSC1 
      or TSC2 reduced the expression of nesfatin-1/NUCB2 in Min-6 cells, suggesting a 
      direct effect of mTOR signaling. CONCLUSION: Gastric mTOR is a gastric energy 
      sensor whose activity is linked to the regulation of gastric nesfatin-1/NUCB2.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Li, Ziru
AU  - Li Z
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Peking University, Beijing, China.
FAU - Xu, Geyang
AU  - Xu G
FAU - Li, Yin
AU  - Li Y
FAU - Zhao, Jing
AU  - Zhao J
FAU - Mulholland, Michael W
AU  - Mulholland MW
FAU - Zhang, Weizhen
AU  - Zhang W
LA  - eng
GR  - R01 DK043225/DK/NIDDK NIH HHS/United States
GR  - R01 DK054032/DK/NIDDK NIH HHS/United States
GR  - R37 DK043225/DK/NIDDK NIH HHS/United States
GR  - R01DK043225/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120403
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nucb1 protein, mouse)
RN  - 0 (Nucleobindins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tsc1 protein, mouse)
RN  - 0 (Tsc2 protein, mouse)
RN  - 0 (Tuberous Sclerosis Complex 1 Protein)
RN  - 0 (Tuberous Sclerosis Complex 2 Protein)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Calcium-Binding Proteins/genetics/*metabolism
MH  - Cell Line
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Diet, High-Fat/adverse effects
MH  - Endocrine Cells/metabolism
MH  - Enzyme Activation
MH  - Fasting/metabolism
MH  - Gastric Mucosa/*metabolism/pathology
MH  - Gene Expression Regulation
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Nucleobindins
MH  - Phosphorylation
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Sirolimus/pharmacology
MH  - TOR Serine-Threonine Kinases/antagonists & inhibitors/genetics/*metabolism
MH  - Tuberous Sclerosis Complex 1 Protein
MH  - Tuberous Sclerosis Complex 2 Protein
MH  - Tumor Suppressor Proteins/metabolism
PMC - PMC3711577
EDAT- 2012/04/18 06:00
MHDA- 2012/08/10 06:00
PMCR- 2013/04/01
CRDT- 2012/04/18 06:00
PHST- 2012/01/16 00:00 [accepted]
PHST- 2012/04/18 06:00 [entrez]
PHST- 2012/04/18 06:00 [pubmed]
PHST- 2012/08/10 06:00 [medline]
PHST- 2013/04/01 00:00 [pmc-release]
AID - 000338503 [pii]
AID - cpb-0029-0493 [pii]
AID - 10.1159/000338503 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2012;29(3-4):493-500. doi: 10.1159/000338503. Epub 2012 Apr 
      3.