PMID- 22508062
OWN - NLM
STAT- MEDLINE
DCOM- 20120809
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 29
IP  - 3-4
DP  - 2012
TI  - IL-6 production stimulated by CD14(+) monocytes-paracrined IL-1beta does not 
      contribute to the immunosuppressive activity of human umbilical cord mesenchymal 
      stem cells.
PG  - 551-60
LID - 10.1159/000338509 [doi]
AB  - BACKGROUND/AIMS: Human umbilical cord mesenchymal stem cells (hUC-MSCs) possess 
      immunosuppressive activities but the mechanisms of such activities are not fully 
      understood. Here, we investigated the role of IL-6, one of the characteristic 
      factors of MSCs, in the immunoregulating effect of hUC-MSCs on CD4(+) T 
      lymphocytes. METHODS: The condition media from human peripheral blood mononuclear 
      cells (hPBMCs) or CD14+/- cell were tested if stimulating IL-6 production by 
      hUC-MSCs. The related signaling pathway of IL-6, and the immunosuppressive 
      activity of IL-6 on CD4(+) T lymphocytes were studied. RESULT: IL-6 production 
      was dramatically increased by hUC-MSCs when co-culturing with resting or 
      activated hPBMCs. CD14(+) monocytes-paracrined IL-1beta promoted the secretion of 
      IL-6 by hUC-MSCs via JNK and NF-kappaB signaling pathway. Blocking of PGE2 synthesis 
      did not affect the secretion of IL-6, anti-IL-6 antibody was not able to reverse 
      hUC-MSCs-mediated inhibition on CD4(+) T lymphocytes. IL-6 did not mediate the 
      suppressive activity of IL-1beta-hUC-MSCs- PGE2 on CD4(+) T cell. CONCLUSION: 
      CD14(+) monocytes-paracrined IL-1beta promotes IL-6 secretion by hUC-MSCs through 
      activating JNK and NF-kappaB signaling pathway. However, increased IL-6 production 
      does not contribute to immunosuppressive activity of IL-1beta-hUC-MSCs- PGE2 on 
      CD4(+) T cells.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Wang, Ding
AU  - Wang D
AD  - The State Key Laboratory of Experimental Hematology, Institute of Hematology and 
      Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union 
      of Medical College, Tianjin, China.
FAU - Ji, Yue Ru
AU  - Ji YR
FAU - Chen, Ke
AU  - Chen K
FAU - Du, Wei Ting
AU  - Du WT
FAU - Yang, Zhou Xin
AU  - Yang ZX
FAU - Han, Zhi-Bo
AU  - Han ZB
FAU - Chi, Ying
AU  - Chi Y
FAU - Liang, Lu
AU  - Liang L
FAU - Bayard, Francis
AU  - Bayard F
FAU - Han, Zhong Chao
AU  - Han ZC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120403
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (IL1B protein, human)
RN  - 0 (IL6 protein, human)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Nitrobenzenes)
RN  - 0 (Sulfonamides)
RN  - 123653-11-2 (N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/drug effects/*immunology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Culture Media, Conditioned
MH  - Dinoprostone/antagonists & inhibitors/immunology/pharmacology
MH  - Humans
MH  - Immunophenotyping
MH  - Immunosuppressive Agents/immunology/*pharmacology
MH  - Interleukin-1beta/*immunology/metabolism/pharmacology
MH  - Interleukin-6/*immunology/metabolism/pharmacology
MH  - Leukocytes, Mononuclear/*immunology
MH  - Lipopolysaccharide Receptors/immunology
MH  - MAP Kinase Signaling System
MH  - Macrophage Activation
MH  - Mesenchymal Stem Cells/*immunology
MH  - Nitrobenzenes/pharmacology
MH  - Sulfonamides/pharmacology
MH  - Umbilical Cord/cytology/immunology
EDAT- 2012/04/18 06:00
MHDA- 2012/08/10 06:00
CRDT- 2012/04/18 06:00
PHST- 2012/02/02 00:00 [accepted]
PHST- 2012/04/18 06:00 [entrez]
PHST- 2012/04/18 06:00 [pubmed]
PHST- 2012/08/10 06:00 [medline]
AID - 000338509 [pii]
AID - 10.1159/000338509 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2012;29(3-4):551-60. doi: 10.1159/000338509. Epub 2012 Apr 
      3.