PMID- 22508471
OWN - NLM
STAT- MEDLINE
DCOM- 20120828
LR  - 20140731
IS  - 1423-0313 (Electronic)
IS  - 0031-7012 (Linking)
VI  - 89
IP  - 3-4
DP  - 2012
TI  - Genistein attenuated allergic airway inflammation by modulating the transcription 
      factors T-bet, GATA-3 and STAT-6 in a murine model of asthma.
PG  - 229-36
LID - 10.1159/000337180 [doi]
AB  - BACKGROUND: Genistein, a flavonoid in legumes and some herbal medicines, has 
      various biological actions. Previous studies have shown that genistein decreased 
      airway inflammation in allergic asthma. However, studies on how genistein affects 
      immunoreactions in asthma are very limited. OBJECTIVE: It was the aim of this 
      study to investigate the effect of genistein on T helper 1 (Th1)/Th2 cytokines in 
      a murine asthma model and to explore its underlying mechanisms. METHODS: The 
      asthma model was set up both in vivo and in vitro: the mice were divided into 
      four groups in vivo, i.e. control group, ovalbumin-sensitized (OVA) group, Gen20 
      group (20 mg/kg genistein) and Gen40 group (40 mg/kg genistein), and into three 
      groups in vitro, i.e. control group, OVA group, genistein group. Changes in lung 
      histology were observed and concentrations of interleukin-4, interleukin-5 and 
      interferon-gamma in bronchoalveolar lavage fluid and serum were measured by 
      enzyme-linked immunosorbent assay. The mRNA expression of GATA binding protein 3 
      (GATA-3), signal transducer and activator of transcription 6 (STAT-6) and T-box 
      transcription factor (T-bet) in the lungs and CD4+ T cells of each group were 
      detected by real-time PCR and the corresponding proteins were detected by Western 
      blot. RESULTS: The results showed that genistein attenuated OVA-induced airway 
      inflammation, decreased Th2-type cytokines and increased Th1-type cytokines. 
      Additionally, our data suggested that genistein may modulate the Th1/Th2 reaction 
      by inhibiting GATA-3 and STAT-6 production while increasing T-bet production. 
      CONCLUSION: Genistein may modulate the immunomodulatory actions caused by Th1/Th2 
      cytokines in asthma, at least partially, by the down-regulation of GATA-3 and 
      STAT-6 and the up-regulation of T-bet.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Gao, Fei
AU  - Gao F
AD  - Emergency Department, Wuxi People's Hospital, Nanjing Medical University, Wuxi, 
      PR China.
FAU - Wei, Dong
AU  - Wei D
FAU - Bian, Tao
AU  - Bian T
FAU - Xie, Ping
AU  - Xie P
FAU - Zou, Jian
AU  - Zou J
FAU - Mu, Huijun
AU  - Mu H
FAU - Zhang, Bin
AU  - Zhang B
FAU - Zhou, Xiaoyan
AU  - Zhou X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120413
PL  - Switzerland
TA  - Pharmacology
JT  - Pharmacology
JID - 0152016
RN  - 0 (Allergens)
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (Gata3 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (STAT6 Transcription Factor)
RN  - 0 (Stat6 protein, mouse)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor TBX21)
RN  - 9006-59-1 (Ovalbumin)
RN  - DH2M523P0H (Genistein)
SB  - IM
MH  - Allergens/immunology
MH  - Animals
MH  - Anti-Allergic Agents/*therapeutic use
MH  - Anti-Inflammatory Agents/*therapeutic use
MH  - Asthma/*drug therapy/immunology/pathology
MH  - Bronchoalveolar Lavage Fluid/immunology
MH  - CD4-Positive T-Lymphocytes/drug effects/immunology
MH  - Cells, Cultured
MH  - Cytokines/blood/immunology
MH  - Disease Models, Animal
MH  - GATA3 Transcription Factor/genetics/immunology
MH  - Genistein/*therapeutic use
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/immunology
MH  - RNA, Messenger/immunology
MH  - STAT6 Transcription Factor/genetics/immunology
MH  - T-Box Domain Proteins/genetics/immunology
EDAT- 2012/04/18 06:00
MHDA- 2012/08/29 06:00
CRDT- 2012/04/18 06:00
PHST- 2011/12/06 00:00 [received]
PHST- 2012/02/06 00:00 [accepted]
PHST- 2012/04/18 06:00 [entrez]
PHST- 2012/04/18 06:00 [pubmed]
PHST- 2012/08/29 06:00 [medline]
AID - 000337180 [pii]
AID - 10.1159/000337180 [doi]
PST - ppublish
SO  - Pharmacology. 2012;89(3-4):229-36. doi: 10.1159/000337180. Epub 2012 Apr 13.