PMID- 22509803
OWN - NLM
STAT- MEDLINE
DCOM- 20120803
LR  - 20191210
IS  - 1938-5404 (Electronic)
IS  - 0033-7587 (Linking)
VI  - 177
IP  - 5
DP  - 2012 May
TI  - A modified system for analyzing ionizing radiation-induced chromosome 
      abnormalities.
PG  - 533-8
AB  - The analysis of dicentric chromosomes in human peripheral blood lymphocytes 
      (PBLs) by Giemsa staining is the most established method for biological 
      dosimetry. However, this method requires a well-trained person because of the 
      difficulty in detecting aberrations rapidly and accurately. Here, we applied a 
      fluorescence in situ hybridization (FISH) technique, using telomere and 
      centromere peptide nucleic acid (PNA) probes, to solve the problem of biological 
      dosimetry in radiation emergency medicine. A comparison by a well-trained 
      observer found that FISH analysis of PBLs for the dose estimation was more 
      accurate than the conventional Giemsa analysis, especially in samples irradiated 
      at high doses. These results show that FISH analysis with centromeric/telomeric 
      PNA probes could become the standard method for biological dosimetry in radiation 
      emergency medicine.
FAU - Shi, Lin
AU  - Shi L
AD  - Department of Cellular Biology, Research Institute for Radiation Biology and 
      Medicine, Hiroshima University, Hiroshima 734-8553, Japan.
FAU - Fujioka, Kurumi
AU  - Fujioka K
FAU - Sun, Jiying
AU  - Sun J
FAU - Kinomura, Aiko
AU  - Kinomura A
FAU - Inaba, Toshiya
AU  - Inaba T
FAU - Ikura, Tsuyoshi
AU  - Ikura T
FAU - Ohtaki, Megu
AU  - Ohtaki M
FAU - Yoshida, Mitsuaki
AU  - Yoshida M
FAU - Kodama, Yoshiaki
AU  - Kodama Y
FAU - Livingston, Gordon K
AU  - Livingston GK
FAU - Kamiya, Kenji
AU  - Kamiya K
FAU - Tashiro, Satoshi
AU  - Tashiro S
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120417
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
RN  - 0 (Azure Stains)
RN  - 0 (Molecular Probes)
RN  - 0 (Peptide Nucleic Acids)
SB  - IM
MH  - Adult
MH  - Azure Stains
MH  - Centromere/ultrastructure
MH  - Chromosome Aberrations/*radiation effects
MH  - Chromosome Breakage/radiation effects
MH  - Chromosomes, Human/*radiation effects/ultrastructure
MH  - Dose-Response Relationship, Radiation
MH  - Emergency Medicine/methods
MH  - Female
MH  - Gamma Rays/adverse effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - In Vitro Techniques
MH  - Lymphocytes/radiation effects/ultrastructure
MH  - Male
MH  - Metaphase
MH  - Middle Aged
MH  - *Molecular Probes
MH  - *Peptide Nucleic Acids/genetics
MH  - Radiometry/*methods
MH  - Ring Chromosomes
MH  - Staining and Labeling
MH  - Telomere/ultrastructure
EDAT- 2012/04/19 06:00
MHDA- 2012/08/04 06:00
CRDT- 2012/04/19 06:00
PHST- 2012/04/19 06:00 [entrez]
PHST- 2012/04/19 06:00 [pubmed]
PHST- 2012/08/04 06:00 [medline]
AID - 10.1667/RR2849.1 [pii]
AID - 10.1667/rr2849.1 [doi]
PST - ppublish
SO  - Radiat Res. 2012 May;177(5):533-8. doi: 10.1667/rr2849.1. Epub 2012 Apr 17.