PMID- 22509848
OWN - NLM
STAT- MEDLINE
DCOM- 20120803
LR  - 20120418
IS  - 1532-2297 (Electronic)
IS  - 1082-6068 (Linking)
VI  - 42
IP  - 3
DP  - 2012
TI  - Cell separation by countercurrent centrifugal elutriation: recent developments.
PG  - 217-33
LID - 10.1080/10826068.2011.602799 [doi]
AB  - Countercurrent centrifugal elutriation (CCE) is a cell separation technique that 
      separates particles predominantly according to their size, and to some degree 
      according to their specific density, without a need for antibodies or ligands 
      tagging cell surfaces. The principles of this technique have been known for half 
      a century. Still, numerous recent publications confirmed that CCE is a valuable 
      supplement to current cell separation technology. It is mainly applied when 
      homogeneous populations of cells, which mirror an in vivo situation, are required 
      for answering scientific questions or for clinical transplantation, while 
      antibodies or ligands suitable for cell isolation are not available. Currently, 
      new technical developments are expanding its application toward fractionation of 
      healthy and malignant tissue cells and the preparation of dendritic cells for 
      immunotherapy.
FAU - Grosse, Jirka
AU  - Grosse J
AD  - Department of Nuclear Medicine, University of Regensburg, Regensburg, Germany.
FAU - Meier, Karl
AU  - Meier K
FAU - Bauer, Thomas J
AU  - Bauer TJ
FAU - Eilles, Christoph
AU  - Eilles C
FAU - Grimm, Daniela
AU  - Grimm D
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prep Biochem Biotechnol
JT  - Preparative biochemistry & biotechnology
JID - 9607037
SB  - IM
MH  - Apoptosis
MH  - Blood Cells/cytology
MH  - Bone Marrow Cells/cytology
MH  - Cell Count
MH  - Cell Cycle
MH  - Cell Separation/*instrumentation/*methods
MH  - Cell Size
MH  - Centrifugation, Density Gradient/instrumentation/*methods
MH  - Humans
MH  - Particle Size
MH  - Sensitivity and Specificity
EDAT- 2012/04/19 06:00
MHDA- 2012/08/04 06:00
CRDT- 2012/04/19 06:00
PHST- 2012/04/19 06:00 [entrez]
PHST- 2012/04/19 06:00 [pubmed]
PHST- 2012/08/04 06:00 [medline]
AID - 10.1080/10826068.2011.602799 [doi]
PST - ppublish
SO  - Prep Biochem Biotechnol. 2012;42(3):217-33. doi: 10.1080/10826068.2011.602799.