PMID- 22510010
OWN - NLM
STAT- MEDLINE
DCOM- 20120716
LR  - 20131121
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Linking)
VI  - 37
IP  - 5
DP  - 2012 May
TI  - Effect of curcumin on miRNA expression in human Y79 retinoblastoma cells.
PG  - 421-8
LID - 10.3109/02713683.2011.647224 [doi]
AB  - PURPOSE: Retinoblastoma (RB) is the most common intraocular malignancy in 
      children. Deregulation of several miRNAs has been identified in RB, suggesting a 
      potential role in tumorigenesis. Recent evidence suggests that many dietary 
      components like folate, retinoids and curcumin act as potential 
      anticancer/antiproliferative agents by regulating the expression of miRNA. In 
      this study, we investigated the effect of phenolic compound curcumin on miRNA 
      expression in Y79 RB cells. MATERIALS AND METHODS: We analyzed the expression 
      profile of miRNA by microarray analysis and quantitative real-time polymerase 
      chain reaction (qRT-PCR) in curcumin-treated Y79 RB cells. Transfection of miR-22 
      was performed using Lipofectamine 2000. Cell viability, in vitro scratch 
      migration assay, prediction of miRNA targets and Western blot analysis were 
      performed to determine the biological function of miR-22 in Y79 RB cells. 
      RESULTS: In Y79 RB cells treated with curcumin, 5 human miRNAs were upregulated 
      and 16 were downregulated as detected with the miRNA microarray analysis. miR-22, 
      a tumor-suppressor miRNA was one of the miRNA which was upregulated by curcumin. 
      Transfected miR-22 Y79 cells inhibited the cell proliferation and reduced the 
      migration, and erythoblastic leukemia viral oncogene homolog 3 (Erbb3) was 
      confirmed to be the target gene of miR-22. CONCLUSION: These observations suggest 
      that curcumin modulate the miRNA expression profile, thereby exerting its 
      anticancer effects on RB cells.
FAU - Sreenivasan, Seethalakshmi
AU  - Sreenivasan S
AD  - L&T Department of Ocular Pathology, Vision Research Foundation, Sankara 
      Nethralaya, Nungambakkam, Chennai, Tamil Nadu, India.
FAU - Thirumalai, Karthiyaini
AU  - Thirumalai K
FAU - Danda, Ravikanth
AU  - Danda R
FAU - Krishnakumar, Subramanian
AU  - Krishnakumar S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Antineoplastic Agents)
RN  - 0 (MicroRNAs)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Blotting, Western
MH  - Cell Survival/drug effects
MH  - Curcumin/*pharmacology
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Genes, Retinoblastoma/drug effects/*genetics
MH  - Humans
MH  - MicroRNAs/*biosynthesis/drug effects/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Retina/drug effects/metabolism/*pathology
MH  - Retinal Neoplasms/drug therapy/*genetics/metabolism
MH  - Retinoblastoma/drug therapy/*genetics/metabolism
MH  - Tumor Cells, Cultured
EDAT- 2012/04/19 06:00
MHDA- 2012/07/17 06:00
CRDT- 2012/04/19 06:00
PHST- 2012/04/19 06:00 [entrez]
PHST- 2012/04/19 06:00 [pubmed]
PHST- 2012/07/17 06:00 [medline]
AID - 10.3109/02713683.2011.647224 [doi]
PST - ppublish
SO  - Curr Eye Res. 2012 May;37(5):421-8. doi: 10.3109/02713683.2011.647224.