PMID- 22510410
OWN - NLM
STAT- MEDLINE
DCOM- 20120709
LR  - 20120507
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 421
IP  - 2
DP  - 2012 May 4
TI  - Regulation of the bovine SCD5 promoter by EGR2 and SREBP1.
PG  - 375-9
LID - 10.1016/j.bbrc.2012.04.023 [doi]
AB  - In rodents, the transcription factors early growth response 2 (EGR2) and sterol 
      regulatory element binding protein 1a (SREBP1a) regulate transcription of the 
      stearoyl-CoA desaturase 2 (SCD2) gene during peripheral nerve myelination, which 
      may be important for synthesis of the lipid component of myelin. Most non-rodent 
      genomes do not contain the SCD2 gene, but rather express SCD5 in brain and 
      nervous tissues. In this paper, we asked whether bovine SCD5 is regulated in a 
      similar manner to rodent SCD2. Expression of EGR2 did not result in an increase 
      in endogenous SCD5 mRNA expression in JEG3 cells, but did result in activation of 
      truncated bovine SCD5 promoter luciferase reporter constructs. Similar results 
      were obtained with expression of the active form of SREBP1a; however, unlike 
      rodent SCD2, there was no synergistic activation of the bovine SCD5 promoter 
      reporters when EGR2 and SREBP1a were co-expressed. Mutation of the putative EGR2 
      binding site in the SCD5 promoter abolished activation by SREBP1a, suggesting 
      that EGR2 and SREBP1a bind to the same site in the SCD5 promoter. Finally, we 
      have identified a region of the bovine SCD5 promoter between 505 and 305 base 
      pairs upstream of the transcriptional start site that appears to be important for 
      maintaining basal levels of transcription of this gene. While it appears that 
      there are some differences between the regulation of rodent SCD2 and bovine SCD5, 
      the promoters of both genes can be activated by EGR2 and SREBP1a. This is the 
      first report of potential regulators of SCD5 transcription.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Lengi, Andrea J
AU  - Lengi AJ
AD  - Department of Dairy Science, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA 24061-0315, United States.
FAU - Corl, Benjamin A
AU  - Corl BA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120409
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Early Growth Response Protein 2)
RN  - 0 (Sterol Regulatory Element Binding Protein 1)
RN  - EC 1.14.19.1 (Stearoyl-CoA Desaturase)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Line, Tumor
MH  - Early Growth Response Protein 2/genetics/*metabolism
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Mutation
MH  - *Promoter Regions, Genetic
MH  - Stearoyl-CoA Desaturase/*genetics
MH  - Sterol Regulatory Element Binding Protein 1/genetics/*metabolism
MH  - Transcription, Genetic
EDAT- 2012/04/19 06:00
MHDA- 2012/07/10 06:00
CRDT- 2012/04/19 06:00
PHST- 2012/03/07 00:00 [received]
PHST- 2012/04/03 00:00 [accepted]
PHST- 2012/04/19 06:00 [entrez]
PHST- 2012/04/19 06:00 [pubmed]
PHST- 2012/07/10 06:00 [medline]
AID - S0006-291X(12)00659-6 [pii]
AID - 10.1016/j.bbrc.2012.04.023 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 May 4;421(2):375-9. doi: 
      10.1016/j.bbrc.2012.04.023. Epub 2012 Apr 9.