PMID- 22510824
OWN - NLM
STAT- MEDLINE
DCOM- 20121217
LR  - 20190606
IS  - 1678-8060 (Electronic)
IS  - 0074-0276 (Linking)
VI  - 107
IP  - 3
DP  - 2012 May
TI  - Production of TNF-alpha, nitric oxide and hydrogen peroxide by macrophages from mice 
      with paracoccidioidomycosis that were fed a linseed oil-enriched diet.
PG  - 303-9
LID - S0074-02762012000300003 [pii]
AB  - Omega-3 polyunsaturated fatty acids (n-3 PUFA) can modulate the immune system and 
      their primary effect is on macrophage function. Paracoccidioidomycosis (PCM) is 
      an endemic systemic mycosis in Latin America that is caused by the dimorphic 
      fungus Paracoccidioides brasiliensis (Pb). Macrophages are the main defence 
      against this pathogen and have microbicidal activity that is dependent on 
      interferon-Gamma and tumour necrosis factor (TNF)-alpha. These cytokines stimulate the 
      synthesis of nitric oxide (NO) and hydrogen peroxide (H(2)O(2)), leading to the death 
      of the fungus. To study the effect of n-3 PUFA on the host immune response during 
      experimental PCM, macrophages that were obtained from animals infected with Pb18 
      and fed a diet enriched by linseed (LIN) oil were cultured and challenged with 
      the fungus in vitro. The macrophage function was analysed based on the 
      concentrations of TNF-alpha, NO and H(2)O(2). LIN oil seems to influence the production 
      of TNF-alpha during the development of disease. A diet enriched with LIN oil 
      influences the microbicidal activity of the macrophages by inducing the 
      production of cytokines and metabolites such as NO and H(2)O(2), predominantly in the 
      chronic phase of infection.
FAU - Sargi, Sheisa Cyleia
AU  - Sargi SC
AD  - Laboratorio de Imunogenetica, Departamento de Ciencias Basicas da Saude, 
      Universidade Estadual de Maringa, Maringa, PR, Brasil.
FAU - Dalalio, Marcia Machado de Oliveira
AU  - Dalalio MM
FAU - Visentainer, Jesui Vergilio
AU  - Visentainer JV
FAU - Bezerra, Rafael Campos
AU  - Bezerra RC
FAU - Perini, Joao Angelo de Lima
AU  - Perini JA
FAU - Stevanato, Flavia Braidotti
AU  - Stevanato FB
FAU - Visentainer, Jeane Eliete Laguila
AU  - Visentainer JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Mem Inst Oswaldo Cruz
JT  - Memorias do Instituto Oswaldo Cruz
JID - 7502619
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 8001-26-1 (Linseed Oil)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - Colony Count, Microbial
MH  - Fatty Acids, Omega-3/*administration & dosage
MH  - Hydrogen Peroxide/*metabolism
MH  - Linseed Oil/*administration & dosage
MH  - Macrophage Activation
MH  - Macrophages, Peritoneal/*immunology/microbiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nitric Oxide/*biosynthesis
MH  - Paracoccidioidomycosis/*immunology
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
EDAT- 2012/04/19 06:00
MHDA- 2012/12/18 06:00
CRDT- 2012/04/19 06:00
PHST- 2011/02/16 00:00 [received]
PHST- 2012/02/01 00:00 [accepted]
PHST- 2012/04/19 06:00 [entrez]
PHST- 2012/04/19 06:00 [pubmed]
PHST- 2012/12/18 06:00 [medline]
AID - S0074-02762012000300003 [pii]
AID - 10.1590/s0074-02762012000300003 [doi]
PST - ppublish
SO  - Mem Inst Oswaldo Cruz. 2012 May;107(3):303-9. doi: 
      10.1590/s0074-02762012000300003.