PMID- 22512052
OWN - NLM
STAT- MEDLINE
DCOM- 20120613
LR  - 20220318
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 28
IP  - 1
DP  - 2012 Jan
TI  - Effect of GnRHa on apoptosis and release of VEGF in endometrial cell cultures 
      from patients with adenomyosis.
PG  - 72-3
AB  - AIM: To investigate the effect of Gonadotropin-realeasing hormone agonist (GnRHa) 
      on apoptosis and the release of vascular endothelial growth factor (VEGF) in the 
      in vitro eutopic endometrial cell of adenomyosis. METHODS: Biopsy specimens of 
      eutopic endometrium obtained from 32 women with adenomyosis and 20 normal 
      womenwere studied. Cells were cultured with GnRHa for 24 h, 48 h and 72 h in the 
      concentration of 10-7 mol/L and 105 mol/L. Apoptotic ratio was detected by 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and 
      flow cytometry before and after using GnRHa. VEGF concentrations in culture 
      supernatant were detected by commercial enzyme-linked immunosorbent assay 
      (ELISA). RESULTS: (1)Cells in two groups showed cell shrinkage, floating, nucleus 
      concentration, nuclear fragmentation and typical apoptotic bodies. (2) The 
      apoptotic ratio of cultured AM endometrial cell without GnRHa was lower than that 
      in control group and the apoptotic ratio increased with the prolongation of time 
      in two groups(P<0.01). (3)After GnRHa addition, each group showed higher 
      apoptotic ratio and a trend towards higher apoptotic ratio linked to advanced 
      concentration. Furthermore, apoptotic ratio in study group was significantly 
      higher than that in control group at the same time point and same concentration ( 
      P < 0. 01 ). ( 4 ) The concentrations of VEGF in the eutopic endometrial cells of 
      adenomyosis were significantly higher than those in the normal endometrimn. The 
      concentrations of VEGF in the both groups were downregulated by GnRHa in a 
      dose-dependent manner. CONCLUSION: (1) The abnormality of apoptotic ratio of AM 
      endometrial cells may be associated with the AM pathogenesis. GnRHa nmy increase 
      the apoptotic ratio of cultured AM endometrial cells by autocrine or paracrine. 
      (2) VEGF may play an impotant role in the pathogenesis of adenomyosim. GnRHa can 
      directly suppress the survival and growth of ectopic endometrial by decreasing 
      the release of VEGF which was related to the adenomyosis angiogenesis.
FAU - Xiao-xia, Wang
AU  - Xiao-xia W
AD  - Department of Gynecology and Obstetrics, The First Municipal People's Hospital 
      Affiliated to Guangzhou Medical College, Guangzhou, China. wxx605@sohu.com
FAU - Jia-li, Kang
AU  - Jia-li K
FAU - Xue-fei, Shao
AU  - Xue-fei S
FAU - Jia, Yu
AU  - Jia Y
FAU - Ling-hong, Deng
AU  - Ling-hong D
LA  - eng
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 79561-22-1 (LHRH, Ala(6)-Gly(10)-ethylamide-)
SB  - IM
MH  - Adult
MH  - Apoptosis/*drug effects
MH  - Cells, Cultured
MH  - Endometriosis/*metabolism/pathology
MH  - Endometrium/*metabolism/pathology
MH  - Female
MH  - Flow Cytometry
MH  - Gonadotropin-Releasing Hormone/*analogs & derivatives/pharmacology
MH  - Humans
MH  - Middle Aged
MH  - Vascular Endothelial Growth Factor A/*metabolism
EDAT- 2012/04/20 06:00
MHDA- 2012/06/14 06:00
CRDT- 2012/04/20 06:00
PHST- 2012/04/20 06:00 [entrez]
PHST- 2012/04/20 06:00 [pubmed]
PHST- 2012/06/14 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Jan;28(1):72-3.