PMID- 22513876 OWN - NLM STAT- MEDLINE DCOM- 20121203 LR - 20171116 IS - 1097-0010 (Electronic) IS - 0022-5142 (Linking) VI - 92 IP - 12 DP - 2012 Sep TI - Application of Trichoderma harzianum SQR-T037 bio-organic fertiliser significantly controls Fusarium wilt and affects the microbial communities of continuously cropped soil of cucumber. PG - 2465-70 LID - 10.1002/jsfa.5653 [doi] AB - BACKGROUND: The reduction in diversity of the soil microbial community causes the disorder of continuous cropping. The aim of this study was to determine the effects of applying Trichoderma harzianum SQR-T037 bio-organic fertiliser (BIO) on the microbial community in continuously cropped cucumber soil. Four treatments were set: (1) control, where neither seedling nursery soil (N) nor transplanted soil (T) was amended with BIO; (2) N treatment, where nursery soil was amended with BIO (1% w/w) but transplanted soil was not; (3) N + T treatment, where BIO was added to both nursery soil (1% w/w) and transplanted soil (0.5% w/w); (4) uncropped soil, where soil was left uncropped consistently. RESULTS: A disease index of 72.2% was found for the control treatment, while the N and N + T treatments had disease indices of only 25 and 15% respectively. Analysis of the denaturing gradient gel electrophoresis (DGGE) profiles showed that the bacterial communities of the N and N + T treatments were similar to those of the uncropped soil but distinct from those of the control soil. The fungal communities of the N and N + T treatments differed from those of both the uncropped soil and the control. CONCLUSION: Addition of BIO to both the nursery soil and the transplanted soil can diversify the microbial community in continuously cropped cucumber soil and thus effectively control Fusarium wilt of cucumber plants. CI - Copyright (c) 2012 Society of Chemical Industry. FAU - Chen, Li-Hua AU - Chen LH AD - Jiangsu Provincial Key Laboratory of Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China. FAU - Huang, Xin-Qi AU - Huang XQ FAU - Zhang, Feng-Ge AU - Zhang FG FAU - Zhao, Di-Kun AU - Zhao DK FAU - Yang, Xing-Ming AU - Yang XM FAU - Shen, Qi-Rong AU - Shen QR LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20120419 PL - England TA - J Sci Food Agric JT - Journal of the science of food and agriculture JID - 0376334 RN - 0 (Fertilizers) RN - 0 (Soil) SB - IM MH - Agriculture/methods MH - Cucumis sativus/*microbiology/*physiology MH - Fertilizers/*microbiology MH - *Fusarium MH - Plant Diseases/microbiology/*prevention & control MH - Seedlings MH - Soil MH - *Soil Microbiology MH - *Trichoderma EDAT- 2012/04/20 06:00 MHDA- 2012/12/10 06:00 CRDT- 2012/04/20 06:00 PHST- 2011/09/04 00:00 [received] PHST- 2012/02/08 00:00 [revised] PHST- 2012/02/09 00:00 [accepted] PHST- 2012/04/20 06:00 [entrez] PHST- 2012/04/20 06:00 [pubmed] PHST- 2012/12/10 06:00 [medline] AID - 10.1002/jsfa.5653 [doi] PST - ppublish SO - J Sci Food Agric. 2012 Sep;92(12):2465-70. doi: 10.1002/jsfa.5653. Epub 2012 Apr 19.