PMID- 22515088
OWN - NLM
STAT- MEDLINE
DCOM- 20120726
LR  - 20190923
IS  - 1550-7033 (Print)
IS  - 1550-7033 (Linking)
VI  - 8
IP  - 2
DP  - 2012 Apr
TI  - Structural properties induced by the composition of biocompatible 
      phospholipid-based microemulsion and amphotericin B association.
PG  - 350-9
AB  - Anionic microemulsions (MEs) containing soya phosphatidylcholine, Tween-20, 
      sodium oleate as surfactant, and cholesterol as oil phase were investigated as 
      drug carriers for amphotericin B. Depending on the composition of the 
      microemulsion, various structures, which differently interact with amphotericin 
      B, can be formed. The nanostructured systems were characterized by photon 
      correlation spectroscopy, rheological behavior, and polarized light microscopy. 
      The results reveal that the droplet diameters increased with amphotericin B 
      incorporation for all ranges of surfactant and oil phase. For both amphotericin 
      B-unloaded and amphotericin B-loaded microemulsions, the profile of the oil 
      droplet diameter decreased with increasing the surfactant concentration, 
      demonstrating the stabilizing effect of the surfactant. The increase in the oil 
      phase proportions led to the growth of the droplet diameter, clearly 
      demonstrating the limit of the surfactant organization in the oil-water 
      interface. The amphotericim B incorporation into microemulsion increased with the 
      fraction volume of the oil phase and the surfactant concentration reaching a 
      plateau at high contents. This profile could be quantitatively analyzed by the 
      framework of the pseudo-phase model that considers the amphotericim B 
      distribution between the oil and the aqueous phases. The rheological analysis 
      showed a pseudoplastic behavior with little thixotropic characteristic. Under 
      polarized light, the system of interest showed a dark background characteristic 
      of dispersed droplets. However, for both amphotericim B-loaded and amphotericim B 
      unloaded microemulsions, the increase in the O/S ratio led to the formation of 
      ordered structures with lamellar arrangements.
FAU - Franzini, Cristina Maria
AU  - Franzini CM
AD  - Universidade Estadual Paulista, UNESP, Faculdade de Ciencias Farmaceuticas, 
      Programa de Pos-graduacao em Ciencias Farmaceuticas, Rodovia Araraquara-Jau km 
      01, 14801-902, Araraquara, SP, Brazil.
FAU - Pestana, Kelly Christina
AU  - Pestana KC
FAU - Molina, Eduardo Ferreira
AU  - Molina EF
FAU - Scarpa, Maria Virginia
AU  - Scarpa MV
FAU - do Egito, Eryvaldo Socrates Tabosa
AU  - do Egito ES
FAU - de Oliveira, Anselmo Gomes
AU  - de Oliveira AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Nanotechnol
JT  - Journal of biomedical nanotechnology
JID - 101230869
RN  - 0 (Drug Carriers)
RN  - 0 (Emulsions)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Polysorbates)
RN  - 0 (Surface-Active Agents)
RN  - 2UMI9U37CP (Oleic Acid)
RN  - 399SL044HN (osteum)
RN  - 7XU7A7DROE (Amphotericin B)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Amphotericin B/*chemistry
MH  - Cholesterol/chemistry
MH  - Drug Carriers/*chemistry
MH  - Emulsions/*chemistry
MH  - Microscopy
MH  - Nanostructures/*chemistry/ultrastructure
MH  - Oleic Acid/chemistry
MH  - Particle Size
MH  - Phosphatidylcholines/*chemistry
MH  - Polysorbates/chemistry
MH  - Rheology
MH  - Surface-Active Agents/chemistry
EDAT- 2012/04/21 06:00
MHDA- 2012/07/27 06:00
CRDT- 2012/04/21 06:00
PHST- 2012/04/21 06:00 [entrez]
PHST- 2012/04/21 06:00 [pubmed]
PHST- 2012/07/27 06:00 [medline]
AID - 10.1166/jbn.2012.1435 [doi]
PST - ppublish
SO  - J Biomed Nanotechnol. 2012 Apr;8(2):350-9. doi: 10.1166/jbn.2012.1435.