PMID- 22515169
OWN - NLM
STAT- MEDLINE
DCOM- 20140602
LR  - 20220408
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 43
IP  - 1
DP  - 2012 Apr 19
TI  - Characterization of homologous and heterologous adaptive immune responses in 
      porcine reproductive and respiratory syndrome virus infection.
PG  - 30
LID - 10.1186/1297-9716-43-30 [doi]
AB  - The present study characterized the homologous and heterologous immune response 
      in type-I porcine reproductive and respiratory syndrome virus (PRRSV) infection. 
      Two experiments were conducted: in experiment 1, eight pigs were inoculated with 
      PRRSV strain 3262 and 84 days post-inoculation (dpi) they were challenged with 
      either strain 3262 or strain 3267 and followed for the next 14 days (98 dpi). In 
      experiment 2, eight pigs were inoculated with strain 3267 and challenged at 84 
      dpi as above. Clinical course, viremia, humoral response (neutralizing and 
      non-neutralizing antibodies, NA) and virus-specific IFN-gamma responses (ELISPOT) 
      were evaluated all throughout the study. Serum levels of IL-1, IL-6, IL-8, TNF-alpha 
      and TGF-beta were determined (ELISA) after the second challenge. In experiment 1 
      primo-inoculation with strain 3262 induced viremia of </= 28 days, low titres of 
      homologous NA but strong IFN-gamma responses. In contrast, strain 3267 induced longer 
      viremias (up to 56 days), higher NA titres (</= 6 log2) and lower IFN-gamma responses. 
      Inoculation with 3267 produced higher serum IL-8 levels. After the re-challenge 
      at 84 dpi, pigs in experiment 1 developed mostly a one week viremia regardless of 
      the strain used. In experiment 2, neither the homologous nor the heterologous 
      challenge resulted in detectable viremia although PRRSV was present in tonsils of 
      some animals. Homologous re-inoculation with 3267 produced elevated TGF-beta levels 
      in serum for 7-14 days but this did not occur with the heterologous 
      re-inoculation. In conclusion, inoculation with different PRRSV strains result in 
      different virological and immunological outcomes and in different degrees of 
      homologous and heterologous protection.
FAU - Diaz, Ivan
AU  - Diaz I
AD  - Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat 
      Autonoma de Barcelona, 08193, Bellaterra, Barcelona, Spain. enric.mateu@uab.cat.
FAU - Gimeno, Mariona
AU  - Gimeno M
FAU - Darwich, Laila
AU  - Darwich L
FAU - Navarro, Nuria
AU  - Navarro N
FAU - Kuzemtseva, Liudmila
AU  - Kuzemtseva L
FAU - Lopez, Sergio
AU  - Lopez S
FAU - Galindo, Ivan
AU  - Galindo I
FAU - Segales, Joaquim
AU  - Segales J
FAU - Martin, Margarita
AU  - Martin M
FAU - Pujols, Joan
AU  - Pujols J
FAU - Mateu, Enric
AU  - Mateu E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120419
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Cytokines)
RN  - 0 (Viral Vaccines)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/blood
MH  - Antibodies, Viral/blood
MH  - Cytokines/*genetics/metabolism
MH  - Enzyme-Linked Immunospot Assay/veterinary
MH  - *Immunity, Heterologous
MH  - Interferon-gamma/genetics/metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Random Allocation
MH  - Swine
MH  - Viral Vaccines/*immunology
PMC - PMC3403850
EDAT- 2012/04/21 06:00
MHDA- 2014/06/03 06:00
PMCR- 2012/01/01
CRDT- 2012/04/21 06:00
PHST- 2011/10/24 00:00 [received]
PHST- 2012/04/19 00:00 [accepted]
PHST- 2012/04/21 06:00 [entrez]
PHST- 2012/04/21 06:00 [pubmed]
PHST- 2014/06/03 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - 1297-9716-43-30 [pii]
AID - 10.1186/1297-9716-43-30 [doi]
PST - epublish
SO  - Vet Res. 2012 Apr 19;43(1):30. doi: 10.1186/1297-9716-43-30.