PMID- 22516654
OWN - NLM
STAT- MEDLINE
DCOM- 20120716
LR  - 20131121
IS  - 1096-0384 (Electronic)
IS  - 0003-9861 (Linking)
VI  - 522
IP  - 1
DP  - 2012 Jun 1
TI  - Spin probe analysis of microtubules structure and formation.
PG  - 1-8
LID - 10.1016/j.abb.2012.04.002 [doi]
AB  - Microtubules (MTs) control cell replication, material transport and motion in 
      eukaryotic cells, but MT role in several pathologies is still unknown. These 
      functions are related to the MT physico-chemical properties and MT formation mode 
      starting from tubulin molecules. This study describes a new method, based on the 
      computer aided analysis of the electron paramagnetic resonance (EPR) spectra of 
      selected spin probes to obtain structural and dynamical information on tubulins 
      and MTs and the kinetics of MTs formation promoted by guanosine-5'-triphosphate 
      (GTP). It was found that tubulin and MTs avoid radical quenching caused by 
      ethylene glycol tetraacetic acid (EGTA). MT formation showed different kinetics 
      as a function of tubulin concentration. At 5 mg/mL of tubulin, MTs were formed in 
      8 min. These results are also useful for getting information on MT-drug 
      interactions.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Ottaviani, Maria Francesca
AU  - Ottaviani MF
AD  - Department of Earth, Life and Environment Sciences (DiSTeVA)-Localita Crocicchia, 
      University of Urbino, 61029 Urbino, Italy. maria.ottaviani@uniurb.it
FAU - Pregnolato, Massimo
AU  - Pregnolato M
FAU - Cangiotti, Michela
AU  - Cangiotti M
FAU - Fiorani, Luigi
AU  - Fiorani L
FAU - Fattori, Alberto
AU  - Fattori A
FAU - Danani, Andrea
AU  - Danani A
LA  - eng
PT  - Journal Article
DEP - 20120409
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Biopolymers)
RN  - 0 (Spin Labels)
RN  - 0 (Tubulin)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 86-01-1 (Guanosine Triphosphate)
SB  - IM
MH  - Biopolymers/chemistry
MH  - Dimerization
MH  - Egtazic Acid/chemistry
MH  - Electron Spin Resonance Spectroscopy
MH  - Guanosine Triphosphate/chemistry
MH  - Kinetics
MH  - Microtubules/*chemistry
MH  - Models, Molecular
MH  - *Spin Labels
MH  - Tubulin/chemistry
EDAT- 2012/04/21 06:00
MHDA- 2012/07/17 06:00
CRDT- 2012/04/21 06:00
PHST- 2012/01/12 00:00 [received]
PHST- 2012/04/02 00:00 [revised]
PHST- 2012/04/03 00:00 [accepted]
PHST- 2012/04/21 06:00 [entrez]
PHST- 2012/04/21 06:00 [pubmed]
PHST- 2012/07/17 06:00 [medline]
AID - S0003-9861(12)00133-6 [pii]
AID - 10.1016/j.abb.2012.04.002 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2012 Jun 1;522(1):1-8. doi: 10.1016/j.abb.2012.04.002. Epub 
      2012 Apr 9.