PMID- 22522779
OWN - NLM
STAT- MEDLINE
DCOM- 20120914
LR  - 20150514
IS  - 1552-4930 (Electronic)
IS  - 1552-4922 (Linking)
VI  - 81
IP  - 6
DP  - 2012 Jun
TI  - Allogeneic bone marrow grafts with high levels of CD4(+) CD25(+) FoxP3(+) T cells 
      can lead to engraftment failure.
PG  - 476-88
LID - 10.1002/cyto.a.22061 [doi]
AB  - Regulatory CD4(+) CD25(+) FoxP3(+) T cells (T(regs) ) suppress immunological 
      reactions. However, the effect of adding T(regs) to hematopoietic stem cell 
      grafts on recovery and graft versus host disease (GvHD) is unknown. T(regs) from 
      splenocytes of C57Bl/6 and Balb/c wild-type mice were isolated by MACS separation 
      and analyzed by flow cytometry. Using a murine syngeneic transplantation model 
      that clearly distinguishes between donor and host hematopoiesis, we showed that 
      co-transplantation of bone marrow cells (BMCs) with high levels of T(regs) leads 
      to a 100% survival of the mice and accelerates the hematopoietic recovery 
      significantly (full donor chimerism). In allogeneic transplantation, bone marrow 
      and T(regs) co-transplantation were compared to allogeneic bone marrow 
      transplantation with or without the addition of splenocytes. Survival, leukocyte 
      recovery, chimerism at days -2, 19, 33, and 61 for murine CD4, human CD4, 
      HLA-DR3, murine CD3, murine CD8, murine Balb/c-H2K(d) , murine C57Bl/6-H2K(b) , 
      and GvHD appearance were analyzed. Allogeneic bone marrow transplantation 
      requires the addition of splenocytes to reach engraftment. Mice receiving grafts 
      with bone marrow, splenocytes and high levels of allogeneic T(regs) died within 
      28 days (hematopoietic failure). Here, we show also detailed flow cytometric data 
      reagarding analysis of chimerism after transplantation in unique murine 
      hematopoietic stem cell transplantation models. Our findings showed that the 
      syngeneic co-transplantation of CD4(+) , CD25(+) , FoxP3(+) T-cells and BMCs 
      induced a stimulating effect on reconstitution of hematopoiesis after 
      irradiation. However, in the allogeneic setting the co-transplantation of T(regs) 
      aggravates the engraftment of transplanted cells.
CI  - Copyright (c) 2012 International Society for Advancement of Cytometry.
FAU - Fricke, Stephan
AU  - Fricke S
AD  - Department of Immunology, Fraunhofer Institute for Cell Therapy and Immunology 
      (IZI), Leipzig, Germany. stephan.fricke@izi.fraunhofer.de
FAU - Rothe, Katherina
AU  - Rothe K
FAU - Hilger, Nadja
AU  - Hilger N
FAU - Ackermann, M
AU  - Ackermann M
FAU - Oelkrug, Christopher
AU  - Oelkrug C
FAU - Fricke, Christian
AU  - Fricke C
FAU - Schonfelder, Uta
AU  - Schonfelder U
FAU - Niederwieser, Dietger
AU  - Niederwieser D
FAU - Emmrich, Frank
AU  - Emmrich F
FAU - Sack, Ulrich
AU  - Sack U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120420
PL  - United States
TA  - Cytometry A
JT  - Cytometry. Part A : the journal of the International Society for Analytical 
      Cytology
JID - 101235694
SB  - IM
EIN - Cytometry A. 2015 Mar;87(3):279-80
EIN - Cytometry A. 2015 Mar;87(3):279-280. PMID: 25631159
MH  - Animals
MH  - Bone Marrow Transplantation/*methods/mortality
MH  - Chimerism
MH  - Flow Cytometry
MH  - Graft vs Host Disease/immunology/*prevention & control
MH  - Hematopoietic Stem Cell Transplantation/*methods/mortality
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Spleen/cytology/immunology
MH  - Survival Rate
MH  - T-Lymphocytes, Regulatory/immunology/*transplantation
MH  - Transplantation, Homologous/*adverse effects
MH  - *Transplantation, Isogeneic
MH  - Treatment Failure
MH  - Whole-Body Irradiation
EDAT- 2012/04/24 06:00
MHDA- 2012/09/15 06:00
CRDT- 2012/04/24 06:00
PHST- 2011/08/19 00:00 [received]
PHST- 2012/02/08 00:00 [revised]
PHST- 2012/03/29 00:00 [accepted]
PHST- 2012/04/24 06:00 [entrez]
PHST- 2012/04/24 06:00 [pubmed]
PHST- 2012/09/15 06:00 [medline]
AID - 10.1002/cyto.a.22061 [doi]
PST - ppublish
SO  - Cytometry A. 2012 Jun;81(6):476-88. doi: 10.1002/cyto.a.22061. Epub 2012 Apr 20.