PMID- 22522808
OWN - NLM
STAT- MEDLINE
DCOM- 20121002
LR  - 20211021
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Print)
IS  - 1615-9853 (Linking)
VI  - 12
IP  - 7
DP  - 2012 Apr
TI  - An interactome map of the nucleocapsid protein from a highly pathogenic North 
      American porcine reproductive and respiratory syndrome virus strain generated 
      using SILAC-based quantitative proteomics.
PG  - 1015-23
LID - 10.1002/pmic.201100469 [doi]
AB  - Positive strand RNA viruses replicate in the cytoplasm of an infected cell and 
      encode nucleocapsid proteins. These proteins function to promote encapsidation of 
      the RNA genome and virus particle assembly as well as playing potential roles in 
      viral RNA synthesis. Nucleocapsid proteins can also associate with cellular 
      proteins and signaling cascades. The arterivirus nucleocapsid (N) protein is no 
      exception and localizes to both the cytoplasm and the nucleolus in virus-infected 
      cells. This study generated an interactome map of the N protein from a highly 
      virulent North American strain of porcine reproductive and respiratory syndrome 
      virus (PRRSV). This is a major pathogen of swine resulting in significant 
      morbidity and mortality. Crucial to the study was the use of SILAC coupled to 
      affinity purification using GFP-traps and LC-MS/MS. This approach has not been 
      applied before to the investigation of host/viral protein interactomes and this 
      study revealed that the PRRSV N protein interacts with the host cell protein 
      synthesis machinery especially at the level of translation initiation as well as 
      with the RNA post-transcriptional modification machinery. Applications of the 
      dataset can include studies of virus/host interactions and the design of live 
      attenuated recombinant vaccines.
CI  - (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Jourdan, Stefanie S
AU  - Jourdan SS
AD  - Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, 
      University of Leeds, Leeds, UK.
FAU - Osorio, Fernando
AU  - Osorio F
FAU - Hiscox, Julian A
AU  - Hiscox JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Proteins)
SB  - IM
MH  - Blotting, Western
MH  - Chromatography, Liquid
MH  - Isotope Labeling/*methods
MH  - Nucleocapsid Proteins/chemistry/*metabolism
MH  - Porcine respiratory and reproductive syndrome virus/*metabolism
MH  - Protein Interaction Mapping
MH  - *Protein Interaction Maps
MH  - Proteins/chemistry/classification/metabolism
MH  - Proteomics/*methods
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry
PMC - PMC7167637
EDAT- 2012/04/24 06:00
MHDA- 2012/10/04 06:00
PMCR- 2020/04/20
CRDT- 2012/04/24 06:00
PHST- 2012/04/24 06:00 [entrez]
PHST- 2012/04/24 06:00 [pubmed]
PHST- 2012/10/04 06:00 [medline]
PHST- 2020/04/20 00:00 [pmc-release]
AID - PMIC7023 [pii]
AID - 10.1002/pmic.201100469 [doi]
PST - ppublish
SO  - Proteomics. 2012 Apr;12(7):1015-23. doi: 10.1002/pmic.201100469.