PMID- 22524657
OWN - NLM
STAT- MEDLINE
DCOM- 20120816
LR  - 20220331
IS  - 1440-1827 (Electronic)
IS  - 1320-5463 (Linking)
VI  - 62
IP  - 5
DP  - 2012 May
TI  - Establishment of a monoclonal anti-pan HLA class I antibody suitable for 
      immunostaining of formalin-fixed tissue: unusually high frequency of 
      down-regulation in breast cancer tissues.
PG  - 303-8
LID - 10.1111/j.1440-1827.2012.02789.x [doi]
AB  - A novel monoclonal anti-pan human leukocyte antigen (HLA) class I heavy chain 
      antibody, EMR8-5, was established. It could detect HLA-A, -B, and -C antigens in 
      formalin-fixed paraffin embedded tissues. By immunohistochemical staining using 
      the EMR8-5 antibody, various cancer tissues from 246 cases were examined for HLA 
      class I expression. It was found that HLA class I expression was decreased in 20% 
      to 42% of the cases of lung cancer, hepatocellular carcinoma, colon cancer, renal 
      cell carcinoma, and urothelial carcinoma. In contrast, 85% of breast cancer cases 
      had loss of or decreased HLA class I expression. Of the 35 breast cancer cases 
      that had decreased HLA class I heavy chain expression, 33 (94%) also had 
      decreased beta2-microglobulin expression detected by immunohistochemical 
      staining. It was suggested that HLA class I down-regulation might be a common 
      characteristic of breast cancer mostly caused by the down-regulation of 
      beta2-microglobulin expression.
CI  - (c) 2012 The Authors. Pathology International (c) 2012 Japanese Society of Pathology 
      and Blackwell Publishing Asia Pty Ltd.
FAU - Torigoe, Toshihiko
AU  - Torigoe T
AD  - Departments of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan. torigoe@sapmed.ac.jp
FAU - Asanuma, Hiroko
AU  - Asanuma H
FAU - Nakazawa, Emiri
AU  - Nakazawa E
FAU - Tamura, Yasuaki
AU  - Tamura Y
FAU - Hirohashi, Yoshihiko
AU  - Hirohashi Y
FAU - Yamamoto, Eri
AU  - Yamamoto E
FAU - Kanaseki, Takayuki
AU  - Kanaseki T
FAU - Hasegawa, Tadashi
AU  - Hasegawa T
FAU - Sato, Noriyuki
AU  - Sato N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120316
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (beta 2-Microglobulin)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Antibodies, Monoclonal/*immunology
MH  - Antigens, Neoplasm/*immunology
MH  - Biomarkers, Tumor
MH  - Breast Neoplasms/*immunology/pathology
MH  - *Down-Regulation
MH  - Female
MH  - Formaldehyde
MH  - Genes, MHC Class I/immunology
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Tissue Fixation
MH  - beta 2-Microglobulin/metabolism
EDAT- 2012/04/25 06:00
MHDA- 2012/08/17 06:00
CRDT- 2012/04/25 06:00
PHST- 2012/04/25 06:00 [entrez]
PHST- 2012/04/25 06:00 [pubmed]
PHST- 2012/08/17 06:00 [medline]
AID - 10.1111/j.1440-1827.2012.02789.x [doi]
PST - ppublish
SO  - Pathol Int. 2012 May;62(5):303-8. doi: 10.1111/j.1440-1827.2012.02789.x. Epub 
      2012 Mar 16.