PMID- 22525053
OWN - NLM
STAT- MEDLINE
DCOM- 20120912
LR  - 20120521
IS  - 1872-9428 (Electronic)
IS  - 0166-6851 (Linking)
VI  - 184
IP  - 1
DP  - 2012 Jul
TI  - Functional characterizations of malonyl-CoA:acyl carrier protein transacylase 
      (MCAT) in Eimeria tenella.
PG  - 20-8
LID - 10.1016/j.molbiopara.2012.04.002 [doi]
AB  - Eimeria tenella, an apicomplexan parasite in chickens, possesses an apicoplast 
      and its associated metabolic pathways including the Type II fatty acid synthesis 
      (FAS II). Malonyl-CoA:acyl-carry protein transacylase (MCAT) encoded by the fabD 
      gene is one of the essential enzymes in the FAS II system. In the present study, 
      the entire E. tenella MCAT gene (EtfabD) was cloned and sequenced. Immunolabeling 
      located this protein in the apicoplast organelle in coccidial sporozoites. 
      Functional replacement of the fabD gene with amber mutation of E. coli 
      temperature-sensitive LA2-89 strain by E. tenella EtMCAT demonstrated that EcFabD 
      and EtMCAT perform the same biochemical function. The recombinant EtMCAT protein 
      was expressed and its general biochemical features were also determined. An 
      alkaloid natural product corytuberine (CAS: 517-56-6) could specifically inhibit 
      the EtMCAT activity (IC(50)=16.47muM), but the inhibition of parasite growth in 
      vitro by corytuberine was very weak (the predicted MIC(50)=0.65mM).
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Sun, Mingfei
AU  - Sun M
AD  - Institute of Veterinary Medicine, Guangdong Academy of Agricultural Sciences, 
      Guangzhou, China.
FAU - Zhu, Guan
AU  - Zhu G
FAU - Qin, Zonghua
AU  - Qin Z
FAU - Wu, Caiyan
AU  - Wu C
FAU - Lv, Minna
AU  - Lv M
FAU - Liao, Shenquan
AU  - Liao S
FAU - Qi, Nanshan
AU  - Qi N
FAU - Xie, Mingquan
AU  - Xie M
FAU - Cai, Jianping
AU  - Cai J
LA  - eng
SI  - GENBANK/GU936970
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120415
PL  - Netherlands
TA  - Mol Biochem Parasitol
JT  - Molecular and biochemical parasitology
JID - 8006324
RN  - 0 (DNA, Protozoan)
RN  - 0 (Fatty Acids)
RN  - 0 (Protozoan Proteins)
RN  - EC 2.3.1.39 (Acyl-Carrier Protein S-Malonyltransferase)
SB  - IM
MH  - Acyl-Carrier Protein S-Malonyltransferase/*genetics/*metabolism
MH  - Amino Acid Sequence
MH  - Cloning, Molecular
MH  - DNA, Protozoan/chemistry/genetics
MH  - Eimeria tenella/chemistry/*enzymology/*genetics
MH  - Escherichia coli/genetics/metabolism
MH  - Fatty Acids/biosynthesis
MH  - Genetic Complementation Test
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Organelles/chemistry
MH  - Protozoan Proteins/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
EDAT- 2012/04/25 06:00
MHDA- 2012/09/13 06:00
CRDT- 2012/04/25 06:00
PHST- 2011/08/18 00:00 [received]
PHST- 2012/03/30 00:00 [revised]
PHST- 2012/04/05 00:00 [accepted]
PHST- 2012/04/25 06:00 [entrez]
PHST- 2012/04/25 06:00 [pubmed]
PHST- 2012/09/13 06:00 [medline]
AID - S0166-6851(12)00095-3 [pii]
AID - 10.1016/j.molbiopara.2012.04.002 [doi]
PST - ppublish
SO  - Mol Biochem Parasitol. 2012 Jul;184(1):20-8. doi: 
      10.1016/j.molbiopara.2012.04.002. Epub 2012 Apr 15.