PMID- 22526802
OWN - NLM
STAT- MEDLINE
DCOM- 20130729
LR  - 20221207
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 97
IP  - 5
DP  - 2013 Mar
TI  - Dynamics of specific ammonia-oxidizing bacterial populations and nitrification in 
      response to controlled shifts of ammonium concentrations in wastewater.
PG  - 2183-91
LID - 10.1007/s00253-012-4047-7 [doi]
AB  - Ammonia-oxidizing bacteria (AOB) are essential for the nitrification process in 
      wastewater treatment. To retain these slow-growing bacteria in wastewater 
      treatment plants (WWTPs), they are often grown as biofilms, e.g., on nitrifying 
      trickling filters (NTFs) or on carriers in moving bed biofilm reactors (MBBRs). 
      On NTFs, a decreasing ammonium gradient is formed because of the AOB activity, 
      resulting in low ammonium concentrations at the bottom and reduced biomass with 
      depth. To optimize the NTF process, different ammonium feed strategies may be 
      designed. This, however, requires knowledge about AOB population dynamics. Using 
      fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy, 
      we followed biomass changes during 6 months, of three AOB populations on biofilm 
      carriers. These were immersed in aerated MBBR tanks in a pilot plant receiving 
      full-scale wastewater. Tanks were arranged in series, forming a wastewater 
      ammonium gradient mimicking an NTF ammonium gradient. The biomass of one of the 
      dominating Nitrosomonas oligotropha-like populations increased after an ammonium 
      upshift, reaching levels comparable to the high ammonium control in 28 days, 
      whereas a Nitrosomonas europaea-like population increased relatively slowly. The 
      MBBR results, together with competition studies in NTF systems fed with 
      wastewater under controlled ammonium regimes, suggest a differentiation between 
      the two N. oligotropha populations, which may be important for WWTP 
      nitrification.
FAU - Almstrand, R
AU  - Almstrand R
AD  - Department of Chemistry and Molecular Biology/Microbiology, University of 
      Gothenburg, Box 462, SE-40530 Gothenburg, Sweden.
FAU - Lydmark, P
AU  - Lydmark P
FAU - Lindgren, P-E
AU  - Lindgren PE
FAU - Sorensson, F
AU  - Sorensson F
FAU - Hermansson, M
AU  - Hermansson M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120425
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (Waste Water)
RN  - 0 (Water Pollutants)
SB  - IM
MH  - Biomass
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Confocal
MH  - *Nitrification
MH  - Nitrosomonas/*growth & development/metabolism
MH  - Oxidation-Reduction
MH  - Quaternary Ammonium Compounds/*metabolism
MH  - Wastewater/*chemistry/*microbiology
MH  - Water Pollutants/*metabolism
EDAT- 2012/04/25 06:00
MHDA- 2013/07/31 06:00
CRDT- 2012/04/25 06:00
PHST- 2012/01/31 00:00 [received]
PHST- 2012/03/19 00:00 [accepted]
PHST- 2012/03/16 00:00 [revised]
PHST- 2012/04/25 06:00 [entrez]
PHST- 2012/04/25 06:00 [pubmed]
PHST- 2013/07/31 06:00 [medline]
AID - 10.1007/s00253-012-4047-7 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2013 Mar;97(5):2183-91. doi: 
      10.1007/s00253-012-4047-7. Epub 2012 Apr 25.