PMID- 22527849
OWN - NLM
STAT- MEDLINE
DCOM- 20120907
LR  - 20211021
IS  - 1865-3774 (Electronic)
IS  - 0925-5710 (Linking)
VI  - 95
IP  - 5
DP  - 2012 May
TI  - Proliferation and chondrogenic differentiation potential of menstrual blood- and 
      bone marrow-derived stem cells in two-dimensional culture.
PG  - 484-93
LID - 10.1007/s12185-012-1067-0 [doi]
AB  - Menstrual blood is easily accessible, renewable, and inexpensive source of stem 
      cells. In this study, we investigated the chondrogenic differentiation potential 
      of menstrual blood-derived stem cells (MenSCs) compared with that of bone 
      marrow-derived stem cells (BMSCs) in two-dimensional culture. Following 
      characterization of isolated cells, the potential for chondrogenic 
      differentiation of MenSCs and BMSCs was evaluated by immunocytochemical and 
      molecular experiments. MenSCs were strongly positive for mesenchymal stem cell 
      markers in a manner similar to that of BMSCs. In contrast to BMSCs, MenSCs 
      exhibited marked expression of OCT4, and higher proliferative capacity. 
      Differentiated MenSCs showed strong immunoreactivity to a monoclonal antibody 
      against Collagen type 2, in a pattern similar to BMSCs. Accumulation of 
      proteoglycans in differentiated MenSCs was also comparable with that in 
      differentiated BMSCs. However, the mRNA expression patterns as judged by RT-PCR 
      of chondrogenic markers such as Collagen 2A1, Collagen 9A1 and SOX9 in MenSCs 
      were different from those in BMSCs. Given these findings, MenSCs appear to be a 
      unique stem cell population with higher proliferation than and comparable 
      chondrogenic differentiation ability to BMSCs in two-dimensional culture. Much 
      quantitative studies at the molecular level may elucidate the reasons for the 
      observed differences in MenSCs and BMSCs.
FAU - Khanmohammadi, Manijeh
AU  - Khanmohammadi M
AD  - Department of Embryology and Stem Cells, Reproductive Biotechnology Research 
      Center, Avicenna Research Institute, ACECR, Evin, P.O. Box 19615-1177, Tehran, 
      Iran.
FAU - Khanjani, Sayeh
AU  - Khanjani S
FAU - Bakhtyari, Mahsa Sani
AU  - Bakhtyari MS
FAU - Zarnani, Amir Hassan
AU  - Zarnani AH
FAU - Edalatkhah, Haleh
AU  - Edalatkhah H
FAU - Akhondi, Mohammad Mehdi
AU  - Akhondi MM
FAU - Mirzadegan, Ebrahim
AU  - Mirzadegan E
FAU - Kamali, Kourosh
AU  - Kamali K
FAU - Alimoghadam, Kamran
AU  - Alimoghadam K
FAU - Kazemnejad, Somaieh
AU  - Kazemnejad S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120415
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
SB  - IM
MH  - Adult
MH  - Blood Cells/*cytology
MH  - Bone Marrow Cells/*cytology
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation
MH  - Cell Separation/methods
MH  - Chondrocytes/*cytology
MH  - Female
MH  - Humans
MH  - Menstruation
MH  - Stem Cells/*cytology
MH  - Young Adult
EDAT- 2012/04/25 06:00
MHDA- 2012/09/08 06:00
CRDT- 2012/04/25 06:00
PHST- 2011/11/21 00:00 [received]
PHST- 2012/03/22 00:00 [accepted]
PHST- 2012/03/15 00:00 [revised]
PHST- 2012/04/25 06:00 [entrez]
PHST- 2012/04/25 06:00 [pubmed]
PHST- 2012/09/08 06:00 [medline]
AID - 10.1007/s12185-012-1067-0 [doi]
PST - ppublish
SO  - Int J Hematol. 2012 May;95(5):484-93. doi: 10.1007/s12185-012-1067-0. Epub 2012 
      Apr 15.