PMID- 22528396
OWN - NLM
STAT- MEDLINE
DCOM- 20130114
LR  - 20211021
IS  - 1573-6881 (Electronic)
IS  - 0145-479X (Linking)
VI  - 44
IP  - 3
DP  - 2012 Jun
TI  - alpha-Lipoic acid protects 3T3-L1 adipocytes from NYGGF4 (PID1) 
      overexpression-induced insulin resistance through increasing phosphorylation of 
      IRS-1 and Akt.
PG  - 357-63
LID - 10.1007/s10863-012-9440-5 [doi]
AB  - NYGGF4 (also called PID1) was demonstrated that it may be related to the 
      development of obesity-related IR. We aimed in the present study to further 
      elucidate the effects of NYGGF4 on IR and the underlying mechanisms through using 
      alpha-Lipoic acid (LA) treatment, which could facilitate glucose transport and 
      utilization in fully differentiated adipocytes. Our data showed that the LA 
      pretreatment strikingly enhanced insulin-stimulated glucose uptake through 
      increasing GLUT4 translocation to the PM in NYGGF4 overexpression adipocytes. The 
      reactive oxygen species (ROS) levels in NYGGF4 overexpression adipocytes were 
      strikingly enhanced, which could be decreased by the LA pretreatment. NYGGF4 
      overexpression resulted in significant inhibition of tyrosine phosphorylation of 
      IRS-1 and serine phosphorylation of Akt, whereas incubation with LA strongly 
      activated IRS-1 and Akt phosphorylation in NYGGF4 overexpression adipocytes. 
      These results suggest that LA protects 3T3-L1 adipocytes from NYGGF4-induced IR 
      partially through increasing phosphorylation of IRS-1 and Akt and provide 
      evidence that NYGGF4 may be a potential target for the treatment of obesity and 
      obesity-related IR.
FAU - Wang, Yu-mei
AU  - Wang YM
AD  - Department of Child Health, Huai'an Maternity and Child Health Hospital, Huai'an 
      223002, China.
FAU - Lin, Xiao-fei
AU  - Lin XF
FAU - Shi, Chun-mei
AU  - Shi CM
FAU - Lu, Lan
AU  - Lu L
FAU - Qin, Zhen-Ying
AU  - Qin ZY
FAU - Zhu, Guan-zhong
AU  - Zhu GZ
FAU - Cao, Xin-guo
AU  - Cao XG
FAU - Ji, Chen-bo
AU  - Ji CB
FAU - Qiu, Jie
AU  - Qiu J
FAU - Guo, Xi-rong
AU  - Guo XR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120421
PL  - United States
TA  - J Bioenerg Biomembr
JT  - Journal of bioenergetics and biomembranes
JID - 7701859
RN  - 0 (Carrier Proteins)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (NYGGF4 protein, mouse)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Slc2a4 protein, mouse)
RN  - 73Y7P0K73Y (Thioctic Acid)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*drug effects/metabolism
MH  - Animals
MH  - Carrier Proteins/*biosynthesis/genetics/metabolism
MH  - Glucose Transporter Type 4/metabolism
MH  - Insulin Receptor Substrate Proteins/metabolism
MH  - Insulin Resistance/*physiology
MH  - Mice
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Thioctic Acid/*pharmacology
MH  - Transfection
EDAT- 2012/04/25 06:00
MHDA- 2013/01/15 06:00
CRDT- 2012/04/25 06:00
PHST- 2012/02/01 00:00 [received]
PHST- 2012/03/29 00:00 [accepted]
PHST- 2012/04/25 06:00 [entrez]
PHST- 2012/04/25 06:00 [pubmed]
PHST- 2013/01/15 06:00 [medline]
AID - 10.1007/s10863-012-9440-5 [doi]
PST - ppublish
SO  - J Bioenerg Biomembr. 2012 Jun;44(3):357-63. doi: 10.1007/s10863-012-9440-5. Epub 
      2012 Apr 21.