PMID- 22528865
OWN - NLM
STAT- MEDLINE
DCOM- 20120904
LR  - 20181201
IS  - 1559-0283 (Electronic)
IS  - 1085-9195 (Linking)
VI  - 63
IP  - 2
DP  - 2012 Jun
TI  - Biological characteristics and effect of human umbilical cord mesenchymal stem 
      cells (hUC-MSCs) grafting with blood plasma on bone regeneration in rats.
PG  - 171-81
LID - 10.1007/s12013-012-9354-1 [doi]
AB  - We evaluated the biological characteristics/effect of human umbilical 
      cord-derived mesenchymal stem cells (hUC-MSCs) grafting with blood plasma on bone 
      regeneration in rat tibia nonunion. SD rats (142) were randomly divided into four 
      groups: fracture group (positive control); nonunion group (negative control); 
      hUC-MSCs grafting with blood plasma group; and hUC-MSCs grafting with saline 
      group. Rats were administered tetracycline (30 mg/kg) and calcein blue (5 mg/kg) 
      8 days before killing. The animals were killed under deep anesthesia at 4 and 8 
      weeks post fracture for radiological evaluation and 
      histological/immunohistological studies. The hUC-MSCs grafting with blood plasma 
      group was similar to fracture group: the fracture line blurred in 4 weeks and 
      disappeared in 8 weeks postoperatively. Histological/immunohistological studies 
      showed that hUC-MSCs were of low immunogenicity which merged in rat bone tissue, 
      differentiated into osteogenic lineages, and completed the healing of nonunion. 
      After stem cell transplantation, regardless of whether plasma or saline was used, 
      new multi-center bone formation was observed; fracture site density was better in 
      stem cell grafting with blood plasma group. We, therefore, concluded that the 
      biological characteristics of hUC-MSCs-treated nonunion were different from the 
      standard fracture healing process, and the proliferative and localization 
      capacity of hUC-MSCs might benefit from the use of blood plasma.
FAU - Qu, Zhiguo
AU  - Qu Z
AD  - Department of Orthopaedic Surgery, Siping Central Hospital, Siping, Jilin, China.
FAU - Guo, Libin
AU  - Guo L
FAU - Fang, Guojun
AU  - Fang G
FAU - Cui, Zhenghong
AU  - Cui Z
FAU - Guo, Shengnan
AU  - Guo S
FAU - Liu, Ying
AU  - Liu Y
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell Biochem Biophys
JT  - Cell biochemistry and biophysics
JID - 9701934
RN  - 0 (Antigens, CD)
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bone Morphogenetic Protein 2)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Bone Morphogenetic Protein 2/metabolism
MH  - *Bone Regeneration
MH  - Bony Callus/pathology
MH  - Cells, Cultured
MH  - Fractures, Malunited/pathology/*therapy
MH  - Humans
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Radiography
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tibia/diagnostic imaging/pathology/*physiopathology
MH  - Tibial Fractures/pathology/therapy
MH  - Umbilical Cord/*cytology
EDAT- 2012/04/25 06:00
MHDA- 2012/09/05 06:00
CRDT- 2012/04/25 06:00
PHST- 2012/04/25 06:00 [entrez]
PHST- 2012/04/25 06:00 [pubmed]
PHST- 2012/09/05 06:00 [medline]
AID - 10.1007/s12013-012-9354-1 [doi]
PST - ppublish
SO  - Cell Biochem Biophys. 2012 Jun;63(2):171-81. doi: 10.1007/s12013-012-9354-1.