PMID- 22530084
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20120823
LR  - 20240315
IS  - 2008-4447 (Electronic)
IS  - 2008-3289 (Print)
IS  - 2008-3289 (Linking)
VI  - 3
IP  - 4
DP  - 2011 Dec
TI  - Dye labelled monoclonal antibody assay for detection of Toxic Shock Syndrome 
      Toxin -1 from Staphylococcus aureus.
PG  - 170-6
AB  - OBJECTIVE: The aim of study was to develop a rapid assay, dye labelled monoclonal 
      antibody assay (DLMAA), using non-radioactive organic synthetic dyes for 
      identification of Toxic Shock Syndrome Toxin-1 (TSST-1) producing strains of 
      Staphylococcus aureus. MATERIALS AND METHODS: The assay protocol required only 
      two simple steps; addition of TSST-1 antigen to a nitrocellulose membrane and 
      then adding a colloidal dye labelled antibody (D/A) suspension detection reagent. 
      RESULTS: The sensitivity and specificity of the assay was determined relative to 
      positive and negative strains compared to an ELISA assay. Overall 100% agreement 
      was found between both assays. The sensitivity for detection of TSST-1 was 30 ng. 
      CONCLUSION: The DLMAA did not require handling and disposal of radioactive 
      materials. It is a rapid qualitative technique for detection of TSST-1 toxin at 
      room temperature within a short time.
FAU - Javid, Khojasteh V
AU  - Javid KV
AD  - Department of Bacteriology, Guilan University of Medical Sciences, Faculty of 
      Shahid Beheshty, Rasht, Iran.
FAU - Foster, Ha
AU  - Foster H
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Microbiol
JT  - Iranian journal of microbiology
JID - 101518404
PMC - PMC3330179
OTO - NOTNLM
OT  - Colloidal dye
OT  - ELISA
OT  - Monoclonal antibody
OT  - Staphylococcus aureus
OT  - TSST-1
EDAT- 2012/04/25 06:00
MHDA- 2012/04/25 06:01
PMCR- 2011/12/01
CRDT- 2012/04/25 06:00
PHST- 2012/04/25 06:00 [entrez]
PHST- 2012/04/25 06:00 [pubmed]
PHST- 2012/04/25 06:01 [medline]
PHST- 2011/12/01 00:00 [pmc-release]
AID - IJM-3-170 [pii]
PST - ppublish
SO  - Iran J Microbiol. 2011 Dec;3(4):170-6.