PMID- 22531993
OWN - NLM
STAT- MEDLINE
DCOM- 20121017
LR  - 20120730
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 157
IP  - 8
DP  - 2012 Aug
TI  - Molecular characterization of eight segments of Scylla serrata reovirus (SsRV) 
      provides the complete genome sequence.
PG  - 1551-7
LID - 10.1007/s00705-012-1298-7 [doi]
AB  - Scylla serrata reovirus (SsRV) is one of the most prevalent viral pathogens of 
      mud crabs (S. serrata). Of the 12 double-stranded RNA (dsRNA) genomic segments 
      (S1-S12), the three largest (S1-S3) and S7 were sequenced previously and were 
      shown to have no or only low sequence homology to known members within the family 
      Reoviridae. The sequences of the remaining segments, S4-S6 and S8-S12, are 
      reported here. With the exception of S4, all have single open reading frames 
      (ORFs) on their positive strands, and the terminal sequences 5'-AUAAA(U)/(C) 
      (A)/(U)...G(A)/(G) (A)/(U) (A)/(C)AAC(G)/(U)AU-3' are conserved among currently and 
      previously sequenced segments. S4 contains two out-of-phase ORFs on the positive 
      strand, suggesting that this segment is bicistronic. The ORFs of segments S4-S6 
      and S8-S12 have low or no homology to other reovirus genes, with the exception 
      that all of the SsRV segments have high sequence similarity to those of mud crab 
      reovirus (MCRV) and share the same 5'- and 3'-terminal nucleotide sequences, 
      suggesting that the two viruses belong to the same species in the family 
      Reoviridae. Analysis of virion proteins revealed that SsRV contains at least 
      eight structural proteins, with sizes ranging from 25 to 160 kDa.
FAU - Chen, Jigang
AU  - Chen J
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      Ningbo 315100, Zhejiang Province, China. genomic@163.com
FAU - Xiong, Juan
AU  - Xiong J
FAU - Cui, Bojing
AU  - Cui B
FAU - Yang, Jifang
AU  - Yang J
FAU - Li, Wenchen
AU  - Li W
FAU - Mao, Zhijuan
AU  - Mao Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120426
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brachyura/*virology
MH  - *Genome, Viral
MH  - Open Reading Frames
MH  - Phylogeny
MH  - RNA, Viral/analysis
MH  - Reoviridae/*classification/*genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, RNA
MH  - Sequence Homology, Nucleic Acid
MH  - Viral Structural Proteins/chemistry
EDAT- 2012/04/26 06:00
MHDA- 2012/10/18 06:00
CRDT- 2012/04/26 06:00
PHST- 2012/01/26 00:00 [received]
PHST- 2012/02/28 00:00 [accepted]
PHST- 2012/04/26 06:00 [entrez]
PHST- 2012/04/26 06:00 [pubmed]
PHST- 2012/10/18 06:00 [medline]
AID - 10.1007/s00705-012-1298-7 [doi]
PST - ppublish
SO  - Arch Virol. 2012 Aug;157(8):1551-7. doi: 10.1007/s00705-012-1298-7. Epub 2012 Apr 
      26.