PMID- 22532031
OWN - NLM
STAT- MEDLINE
DCOM- 20130304
LR  - 20171116
IS  - 1438-2199 (Electronic)
IS  - 0939-4451 (Linking)
VI  - 43
IP  - 5
DP  - 2012 Nov
TI  - Apelin-APJ induces ICAM-1, VCAM-1 and MCP-1 expression via NF-kappaB/JNK signal 
      pathway in human umbilical vein endothelial cells.
PG  - 2125-36
LID - 10.1007/s00726-012-1298-7 [doi]
AB  - Apelin receptor (APJ) deficiency has been reported to be preventive against 
      atherosclerosis. However, the mechanism of this effect remains unknown. In this 
      study, quantitative real-time RT-PCR, Western blotting and ELISA analyses 
      revealed a significant increase in the expression of intercellular adhesion 
      molecule-1(ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and monocyte 
      chemoattractant protein-1 (MCP-1) in human umbilical vein endothelial cells 
      (HUVECs) treated with apelin. Inhibitors of cellular signal transduction 
      molecules were used to demonstrate involvement of nuclear factor kappa-B (NF-kappaB) 
      and c-Jun N-terminal kinase (JNK) pathways in apelin-APJ-induced activation of 
      adhesion molecules and chemokines. Inhibition of APJ expression by RNA 
      interference abrogated apelin-induced expression of adhesion molecules and 
      chemokines and apelin-stimulated cellular signal transduction in HUVECs. The 
      apelin-APJ system in endothelial cells is involved in the expression of adhesion 
      molecules and chemokines, which are important for the initiation of endothelial 
      inflammation-related atherosclerosis. Therefore, apelin-APJ and the cell 
      signaling pathways activated by this system in endothelial cells may represent 
      targets for therapy of atherosclerosis.
FAU - Lu, Ying
AU  - Lu Y
AD  - Department of Endocrinology, People's Hospital of Zhongshan City, Zhongshan, 
      Guangdong, 528403, People's Republic of China.
FAU - Zhu, Xiao
AU  - Zhu X
FAU - Liang, Gan-Xiong
AU  - Liang GX
FAU - Cui, Rong-Rong
AU  - Cui RR
FAU - Liu, Yuan
AU  - Liu Y
FAU - Wu, Shan-Shan
AU  - Wu SS
FAU - Liang, Qiu-Hua
AU  - Liang QH
FAU - Liu, Guan-Ying
AU  - Liu GY
FAU - Jiang, Yi
AU  - Jiang Y
FAU - Liao, Xiao-Bo
AU  - Liao XB
FAU - Xie, Hui
AU  - Xie H
FAU - Zhou, Hou-De
AU  - Zhou HD
FAU - Wu, Xian-Ping
AU  - Wu XP
FAU - Yuan, Ling-Qing
AU  - Yuan LQ
FAU - Liao, Er-Yuan
AU  - Liao EY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120425
PL  - Austria
TA  - Amino Acids
JT  - Amino acids
JID - 9200312
RN  - 0 (APLN protein, human)
RN  - 0 (APLNR protein, human)
RN  - 0 (Apelin)
RN  - 0 (Apelin Receptors)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Apelin
MH  - Apelin Receptors
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Chemokine CCL2/*genetics/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression Regulation/drug effects
MH  - Human Umbilical Vein Endothelial Cells/cytology/*drug effects/metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/*genetics/metabolism
MH  - Intercellular Signaling Peptides and Proteins/*pharmacology
MH  - JNK Mitogen-Activated Protein Kinases/genetics/metabolism
MH  - NF-kappa B/genetics/metabolism
MH  - RNA, Small Interfering/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, G-Protein-Coupled/antagonists & inhibitors/*genetics/metabolism
MH  - Signal Transduction/drug effects
MH  - Vascular Cell Adhesion Molecule-1/*genetics/metabolism
EDAT- 2012/04/26 06:00
MHDA- 2013/03/05 06:00
CRDT- 2012/04/26 06:00
PHST- 2011/09/07 00:00 [received]
PHST- 2012/04/06 00:00 [accepted]
PHST- 2012/04/26 06:00 [entrez]
PHST- 2012/04/26 06:00 [pubmed]
PHST- 2013/03/05 06:00 [medline]
AID - 10.1007/s00726-012-1298-7 [doi]
PST - ppublish
SO  - Amino Acids. 2012 Nov;43(5):2125-36. doi: 10.1007/s00726-012-1298-7. Epub 2012 
      Apr 25.