PMID- 22534308
OWN - NLM
STAT- MEDLINE
DCOM- 20120820
LR  - 20190923
IS  - 1738-8872 (Electronic)
IS  - 1017-7825 (Linking)
VI  - 22
IP  - 4
DP  - 2012 Apr
TI  - Glyceraldehyde-3-phosphate, a glycolytic intermediate, prevents cells from 
      apoptosis by lowering S-nitrosylation of glyceraldehyde-3-phosphate 
      dehydrogenase.
PG  - 571-3
AB  - Glyceraldehyde-3-phosphate (G-3-P), the substrate of glyceraldehyde-3-phosphate 
      dehydrogenase (GAPDH), is a key intermediate in several metabolic pathways. 
      Recently, we reported that G-3-P directly inhibits caspase-3 activity in a 
      reversible noncompetitive mode, suggesting the intracellular G-3-P level as a 
      cell fate decision factor. It has been known that apoptotic stimuli induce the 
      generation of NO, and NO S-nitrosylates GAPDH at the catalytic cysteine residue, 
      which confers GAPDH the ability to bind to Siah-1, an E3 ubiquitin ligase. The 
      GAPDH-Siah-1 complex is translocated into the nucleus and subsequently triggers 
      the apoptotic process. Here, we clearly showed that intracellular G-3-P protects 
      GAPDH from S-nitrosylation at above a certain level, and consequently maintains 
      the cell survival. In case G-3-P drops below a certain level as a result of 
      exposure to specific stimuli, G-3-P cannot inhibit S-nitrosylation of GAPDH 
      anymore, and consequently GAPDH translocates with Siah-1 into the nucleus. Based 
      on these results, we suggest that G-3-P functions as a molecule switch between 
      cell survival and apoptosis by regulating S-nitrosylation of GAPDH.
FAU - Lee, Sun Young
AU  - Lee SY
AD  - Medical Proteomics Research Center, KRIBB, Daejeon 305-806, Korea.
FAU - Kim, Jeong Hoon
AU  - Kim JH
FAU - Jung, Hyeyun
AU  - Jung H
FAU - Chi, Seung Wook
AU  - Chi SW
FAU - Chung, Sang J
AU  - Chung SJ
FAU - Lee, Chong-Kil
AU  - Lee CK
FAU - Park, Byoung Chul
AU  - Park BC
FAU - Bae, Kwang-Hee
AU  - Bae KH
FAU - Park, Sung Goo
AU  - Park SG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 142-10-9 (Glyceraldehyde 3-Phosphate)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - *Apoptosis
MH  - Caspase 3/metabolism
MH  - Cells/*cytology/enzymology/metabolism
MH  - Glyceraldehyde 3-Phosphate/*metabolism
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*metabolism
MH  - HeLa Cells
MH  - Humans
EDAT- 2012/04/27 06:00
MHDA- 2012/08/21 06:00
CRDT- 2012/04/27 06:00
PHST- 2012/04/27 06:00 [entrez]
PHST- 2012/04/27 06:00 [pubmed]
PHST- 2012/08/21 06:00 [medline]
AID - JMB022-04-20 [pii]
AID - 10.4014/jmb.1110.10010 [doi]
PST - ppublish
SO  - J Microbiol Biotechnol. 2012 Apr;22(4):571-3. doi: 10.4014/jmb.1110.10010.