PMID- 22535762
OWN - NLM
STAT- MEDLINE
DCOM- 20120725
LR  - 20230216
IS  - 1541-6100 (Electronic)
IS  - 0022-3166 (Linking)
VI  - 142
IP  - 6
DP  - 2012 Jun
TI  - Supplementation with cyanidin-3-O-beta-glucoside protects against 
      hypercholesterolemia-mediated endothelial dysfunction and attenuates 
      atherosclerosis in apolipoprotein E-deficient mice.
PG  - 1033-7
LID - 10.3945/jn.112.157701 [doi]
AB  - In this study, we investigated the protective effects of the anthocyanin 
      cyanidin-3-O-beta-glucoside (C3G) on hypercholesterolemia-induced endothelial 
      dysfunction in apoE-deficient (apoE(-/-)) mice. In the prevention study, twenty 
      8-wk-old male apoE(-/-) mice (n = 10/group) were fed a high-fat, cholesterol-rich 
      diet (HCD) or the HCD supplemented with C3G (2 g/kg diet) for 8 wk. The 
      endothelium-dependent relaxation response to acetylcholine in the aortas of the 
      C3G-fed mice was greater compared with those fed the HCD (P < 0.05). The 
      atherosclerotic plaque area in the aortic sinus of mice fed the C3G diet was 
      lowered by 54% compared with those fed the HCD (P < 0.01). Mice fed C3G had 
      greater expression of the ATP-binding cassette transporter G1 (ABCG1) and lower 
      cholesterol, mainly 7-ketocholesterol (7-KC), concentrations than those fed the 
      HCD. Superoxide production and lipid hydroperoxides in aorta were lower in mice 
      fed C3G compared with those fed the HCD. The phosphorylation levels at Ser1177 of 
      endothelial NO synthase (eNOS) and the production of cyclic GMP (cGMP) in aorta 
      were greater in C3G-fed mice than in HCD-fed mice. In the therapy study, 
      apoE(-/-) mice were fed the HCD for 8 wk and then continued to receive the HCD or 
      were switched to the HCD supplemented with C3G (2 g/kg diet) for another 8 wk. 
      The established endothelial dysfunction and atherosclerosis were reversed, 
      accompanied by greater ABCG1 expression in aorta, lower cholesterol and 7-KC 
      concentrations, and greater generation of cGMP in mice fed C3G compared with 
      those fed the HCD. Taken together, our results show that the anthocyanin C3G 
      prevents or reverses hypercholesterolemia-induced endothelial dysfunction by 
      inhibiting cholesterol and 7-oxysterol accumulation in the aorta and the 
      subsequent decrease in superoxide production, thereby preserving eNOS activity 
      and NO bioavailability.
FAU - Wang, Yun
AU  - Wang Y
AD  - Department of Nutrition, Sun Yat-sen University, Guangzhou, Guangdong Province, 
      People's Republic of China
FAU - Zhang, Yuhua
AU  - Zhang Y
FAU - Wang, Xiaoming
AU  - Wang X
FAU - Liu, Yan
AU  - Liu Y
FAU - Xia, Min
AU  - Xia M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120425
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - 0 (Anthocyanins)
RN  - 0 (Apolipoproteins E)
RN  - 0 (Dietary Fats)
RN  - 0 (Glucosides)
RN  - 0 (Hypolipidemic Agents)
RN  - 0 (cyanidin-3-O-beta-glucopyranoside)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Anthocyanins/*pharmacology
MH  - Aorta/drug effects/pathology
MH  - Apolipoproteins E/genetics/*metabolism
MH  - Atherosclerosis/genetics/*prevention & control
MH  - Dietary Fats/administration & dosage/adverse effects
MH  - Dietary Supplements
MH  - Endothelium, Vascular/drug effects/physiopathology
MH  - Glucosides/*pharmacology
MH  - Hypercholesterolemia/drug therapy/*physiopathology
MH  - Hypolipidemic Agents/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Nitric Oxide
MH  - Oxidative Stress
EDAT- 2012/04/27 06:00
MHDA- 2012/07/26 06:00
CRDT- 2012/04/27 06:00
PHST- 2012/04/27 06:00 [entrez]
PHST- 2012/04/27 06:00 [pubmed]
PHST- 2012/07/26 06:00 [medline]
AID - S0022-3166(22)02792-4 [pii]
AID - 10.3945/jn.112.157701 [doi]
PST - ppublish
SO  - J Nutr. 2012 Jun;142(6):1033-7. doi: 10.3945/jn.112.157701. Epub 2012 Apr 25.