PMID- 22537394
OWN - NLM
STAT- MEDLINE
DCOM- 20120802
LR  - 20191210
IS  - 1873-5835 (Electronic)
IS  - 0145-2126 (Linking)
VI  - 36
IP  - 7
DP  - 2012 Jul
TI  - Will a peripheral blood (PB) sample yield the same diagnostic and prognostic 
      cytogenetic data as the concomitant bone marrow (BM) in myelodysplasia?
PG  - 832-40
LID - 10.1016/j.leukres.2012.03.013 [doi]
AB  - In patients with myelodysplastic syndromes (MDS), chromosome anomalies are 
      detected by conventional cytogenetic studies (CCS) and/or interphase fluorescence 
      in situ hybridization (FISH) of bone marrow (BM) samples and provide prognostic 
      and diagnostic information, which can direct therapy. Whether peripheral blood 
      (PB) can be substituted for bone marrow in these cases and can provide the same 
      information remains unknown. Concurrent BM and PB specimens collected from 100 
      patients with recently diagnosed MDS were studied using both CCS and FISH. While 
      68% of BM samples showed an abnormal karyotype by CCS, only 31% of PB samples 
      were abnormal by CCS. In 12% of patients, FISH and CCS were discordant due to the 
      inability of the FISH panel to detect all possible abnormalities. However, only 
      one case (1%) had a cryptic abnormality detected by FISH. BM and PB FISH were 
      discordant in 3% of cases, most likely due to the smaller clone size in PB vs. 
      BM. While PB should not be substituted for BM at diagnosis, it is a viable 
      alternative for monitoring patients using the appropriate FISH probe(s).
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Cherry, Athena M
AU  - Cherry AM
AD  - Stanford Hospitals and Clinics, Palo Alto, CA 94304, USA. acherry@stanfordmed.org
FAU - Slovak, Marilyn L
AU  - Slovak ML
FAU - Campbell, Lynda J
AU  - Campbell LJ
FAU - Chun, Kathy
AU  - Chun K
FAU - Eclache, Virginie
AU  - Eclache V
FAU - Haase, Detlef
AU  - Haase D
FAU - Haferlach, Claudia
AU  - Haferlach C
FAU - Hildebrandt, Barbara
AU  - Hildebrandt B
FAU - Iqbal, Anwar M
AU  - Iqbal AM
FAU - Jhanwar, Suresh C
AU  - Jhanwar SC
FAU - Ohyashiki, Kazuma
AU  - Ohyashiki K
FAU - Sole, Francesc
AU  - Sole F
FAU - Vandenberghe, Peter
AU  - Vandenberghe P
FAU - VanDyke, Daniel L
AU  - VanDyke DL
FAU - Zhang, Yanming
AU  - Zhang Y
FAU - Dewald, Gordon W
AU  - Dewald GW
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20120425
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
SB  - IM
CIN - Leuk Res. 2012 Jul;36(7):806-7. PMID: 22551656
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy, Needle
MH  - Bone Marrow Examination/methods
MH  - Child
MH  - Cytogenetic Analysis/*methods
MH  - Cytogenetics/methods
MH  - Female
MH  - Hematologic Tests/methods
MH  - Humans
MH  - Karyotyping/methods
MH  - Male
MH  - Medical Oncology/*methods
MH  - Middle Aged
MH  - Models, Biological
MH  - Myelodysplastic Syndromes/blood/*diagnosis/pathology
MH  - Prognosis
MH  - Young Adult
EDAT- 2012/04/28 06:00
MHDA- 2012/08/03 06:00
CRDT- 2012/04/28 06:00
PHST- 2011/11/15 00:00 [received]
PHST- 2012/03/13 00:00 [revised]
PHST- 2012/03/14 00:00 [accepted]
PHST- 2012/04/28 06:00 [entrez]
PHST- 2012/04/28 06:00 [pubmed]
PHST- 2012/08/03 06:00 [medline]
AID - S0145-2126(12)00135-X [pii]
AID - 10.1016/j.leukres.2012.03.013 [doi]
PST - ppublish
SO  - Leuk Res. 2012 Jul;36(7):832-40. doi: 10.1016/j.leukres.2012.03.013. Epub 2012 
      Apr 25.