PMID- 22537809
OWN - NLM
STAT- MEDLINE
DCOM- 20120830
LR  - 20120521
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 429
IP  - 1
DP  - 2012 Jul 20
TI  - Influence of N-linked glycosylation of minor proteins of porcine reproductive and 
      respiratory syndrome virus on infectious virus recovery and receptor interaction.
PG  - 1-11
LID - 10.1016/j.virol.2012.03.010 [doi]
AB  - It has been proposed that the N-linked glycan of the minor proteins of porcine 
      reproductive and respiratory syndrome virus (PRRSV) is important for the 
      production of infectious virus. In this study, we showed that N-linked 
      glycosylation of GP2 is not essential for virus viability and none of the 
      individual glycosylation sites in GP3 has a vital effect on the production of 
      infectious virus. Moreover, mutations of single and double glycosylation sites in 
      GP4 are not critically important for infectious virus recovery, triple and 
      quadruple mutations are lethal. The bimolecular fluorescence complementation 
      (BiFC) analysis also showed that GP4, but might be not GP2, is involved in 
      interaction with cellular receptor CD163 and that glycosylation of GP4 might not 
      play a vital role in the interaction with CD163. The study further revealed that 
      none of the N-glycosylation sites in the minor proteins is critical for the 
      susceptibility of mutants to neutralizing antibody.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Wei, Zuzhang
AU  - Wei Z
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
FAU - Tian, Debin
AU  - Tian D
FAU - Sun, Lichang
AU  - Sun L
FAU - Lin, Tao
AU  - Lin T
FAU - Gao, Fei
AU  - Gao F
FAU - Liu, Runxia
AU  - Liu R
FAU - Tong, Guangzhi
AU  - Tong G
FAU - Yuan, Shishan
AU  - Yuan S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120425
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Virus)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/*metabolism
MH  - Antigens, Differentiation, Myelomonocytic/genetics/*metabolism
MH  - Glycosylation
MH  - Mutation
MH  - Porcine Reproductive and Respiratory Syndrome/genetics/*metabolism/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*metabolism
MH  - Protein Binding
MH  - Receptors, Cell Surface/genetics/*metabolism
MH  - Receptors, Virus/genetics/*metabolism
MH  - Swine
MH  - Viral Envelope Proteins/genetics/*metabolism
EDAT- 2012/04/28 06:00
MHDA- 2012/08/31 06:00
CRDT- 2012/04/28 06:00
PHST- 2011/12/04 00:00 [received]
PHST- 2011/12/31 00:00 [revised]
PHST- 2012/03/07 00:00 [accepted]
PHST- 2012/04/28 06:00 [entrez]
PHST- 2012/04/28 06:00 [pubmed]
PHST- 2012/08/31 06:00 [medline]
AID - S0042-6822(12)00167-5 [pii]
AID - 10.1016/j.virol.2012.03.010 [doi]
PST - ppublish
SO  - Virology. 2012 Jul 20;429(1):1-11. doi: 10.1016/j.virol.2012.03.010. Epub 2012 
      Apr 25.