PMID- 22538014
OWN - NLM
STAT- MEDLINE
DCOM- 20120723
LR  - 20200715
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 90
IP  - 21-22
DP  - 2012 Jun 6
TI  - Sphingomyelin synthase 2 over-expression induces expression of aortic 
      inflammatory biomarkers and decreases circulating EPCs in ApoE KO mice.
PG  - 867-73
LID - 10.1016/j.lfs.2012.04.003 [doi]
AB  - AIMS: This study sought to assess the effect of sphingomyelin synthase 2 (SMS2) 
      over-expression on plaque component and endothelial dysfunction in 
      atherosclerosis. MAIN METHODS: We generated recombinant adenovirus vectors 
      containing human SMS2 cDNA (AdV-SMS2) or control gene GFP cDNA (AdV-GFP). Both 
      AdVs were injected (i.v.) into ApoE KO mice to establish SMS2 over-expressing and 
      control mice models, respectively. The mice were fed a high fat diet for 30 days. 
      We then examined their plasma lipid levels, expression levels of aortic 
      inflammatory biomarkers critical for the plaque's stability, and numbers of 
      peripheral endothelial progenitor cells (EPC). KEY FINDINGS: Compared with the 
      control mice, SMS2 over-expression had significantly (1) increased aortic matrix 
      metalloproteinase-2 (MMP-2), monocyte chemoattractant protein-1 (MCP-1), tissue 
      factor (TF) and cyclooxygenase-2 (COX-2) mRNA levels (1.9-fold, 2.2-fold, 
      2.6-fold and 3.2-fold, respectively, P<0.01) and protein levels (2.2-fold, 
      1.9-fold, 1.9-fold and 2.1-fold, respectively, P<0.01); (2) increased MMP-2, 
      COX-2 in situ expression in aortic root (2.6-fold and 2.3-fold, respectively, 
      P<0.01); (3) decreased aortic COX-1 mRNA levels (65%, P<0.01) and protein levels 
      (64%, P<0.01); and (4) decreased CD34/KDR-positive cells (33%, P<0.01), 
      circulating angiogenic cells (CACs) (50%, P<0.05), and colony forming units 
      (CFUs) (40%, P<0.05) in circulation. SIGNIFICANCE: SMS2 over-expression was 
      probably associated with increased expression of aortic inflammatory biomarkers, 
      as well as decreased numbers of CD34/KDR-positive cells, CACs and CFUs in 
      circulation. Therefore, SMS2 over-expression might correlate with endothelial 
      dysfunction and aggravate atherosclerotic plaque instability in ApoE KO mice.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Zhao, Ya-Rui
AU  - Zhao YR
AD  - School of Pharmacy, Fudan University, Shanghai, China.
FAU - Dong, Ji-Bin
AU  - Dong JB
FAU - Li, Yue
AU  - Li Y
FAU - Wu, Man-Ping
AU  - Wu MP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120413
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Apolipoproteins E)
RN  - 0 (Biomarkers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - EC 2.7.8.- (SGMS1 protein, human)
RN  - EC 2.7.8.- (Transferases (Other Substituted Phosphate Groups))
RN  - EC 2.7.8.27 (Sgms2 protein, mouse)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Aorta/physiopathology
MH  - Apolipoproteins E/*genetics
MH  - Atherosclerosis/genetics/*physiopathology
MH  - Biomarkers/metabolism
MH  - DNA, Complementary/genetics
MH  - Disease Models, Animal
MH  - Endothelial Cells/metabolism
MH  - Gene Expression Regulation
MH  - Genetic Vectors
MH  - Humans
MH  - Inflammation/genetics/*physiopathology
MH  - Male
MH  - Membrane Proteins/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Nerve Tissue Proteins/genetics
MH  - Plaque, Atherosclerotic/genetics/*pathology
MH  - Stem Cells/metabolism
MH  - Transferases (Other Substituted Phosphate Groups)/*genetics
EDAT- 2012/04/28 06:00
MHDA- 2012/07/24 06:00
CRDT- 2012/04/28 06:00
PHST- 2011/11/05 00:00 [received]
PHST- 2012/02/22 00:00 [revised]
PHST- 2012/04/03 00:00 [accepted]
PHST- 2012/04/28 06:00 [entrez]
PHST- 2012/04/28 06:00 [pubmed]
PHST- 2012/07/24 06:00 [medline]
AID - S0024-3205(12)00171-3 [pii]
AID - 10.1016/j.lfs.2012.04.003 [doi]
PST - ppublish
SO  - Life Sci. 2012 Jun 6;90(21-22):867-73. doi: 10.1016/j.lfs.2012.04.003. Epub 2012 
      Apr 13.