PMID- 22538453
OWN - NLM
STAT- MEDLINE
DCOM- 20121130
LR  - 20211021
IS  - 1432-1335 (Electronic)
IS  - 0171-5216 (Linking)
VI  - 138
IP  - 9
DP  - 2012 Sep
TI  - A potential probe set of fluorescence in situ hybridization for detection of lung 
      cancer in bronchial brushing specimens.
PG  - 1541-9
LID - 10.1007/s00432-012-1232-0 [doi]
AB  - PURPOSE: The study aims to find candidate probes of fluorescence in situ 
      hybridization (FISH) for detection of lung cancer with bronchial brushings and to 
      evaluate whether the accuracy of diagnosing lung cancer by cytological deviant 
      and genetic abnormalities is greater than that of cytology alone. METHODS: 
      Centromeric enumeration probes (CEPs) for chromosomes 2, 3, 6, 7, 8, 9, 11, 12, 
      and 17 were analyzed using FISH in 74 surgical resection tissues, 32 operative 
      margin tissues without tumor involvement of lung cancer, and 174 bronchial 
      brushings. RESULTS: The aneuploidy rates of the tested probes were 61.7, 89.1, 
      80.0, 92.7, 65.0, 70.4, 66.7, 71.8, 68.9 % in tumor tissues, and 29.3, 58.9, 
      33.3, 69.6, 67.0, 40.3, 38.0, 49.3, 35.1 % in bronchial brushings. The 
      combination of cytology and FISH using the three-probe set for chromosomes 3+7+8 
      significantly improved the sensitivity of bronchial brushing examination for lung 
      cancer detection (P = 0.00003), especially squamous cell carcinoma (SCC), which 
      increased from 78.0 to 98.2 %. The specificity of the 3+7+8 probe set was 94.6 %. 
      Moreover, a high aneuploidy rate of the probe set in bronchial brushings was 
      detected more often in SCCs (P = 0.029) and late-stage non-small-cell lung cancer 
      (NSCLC) (P = 0.044). Kaplan-Meier curves indicated that adenocarcinoma (ADC) 
      patients with high aneuploidy rate of CEP3 in tissue samples exhibited poorer 
      overall survival (P = 0.016). CONCLUSIONS: FISH performed on cytology 
      preparations is useful for confirmation of cancer diagnosis. The three-probe set, 
      3+7+8, has potential value for the detection of SCCs in bronchial brushings.
FAU - Liu, Yi-Zhen
AU  - Liu YZ
AD  - State Key Laboratory of Molecular Oncology, Cancer Institute/Hospital, Peking 
      Union Medical College, Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, 
      Chaoyang District, Beijing, 100021, China.
FAU - Wang, Zhen
AU  - Wang Z
FAU - Fang, Li-Li
AU  - Fang LL
FAU - Li, Lu
AU  - Li L
FAU - Cao, Jian
AU  - Cao J
FAU - Xu, Xin
AU  - Xu X
FAU - Han, Ya-Ling
AU  - Han YL
FAU - Cai, Yan
AU  - Cai Y
FAU - Wang, Liang-Xu
AU  - Wang LX
FAU - Wang, Ming-Rong
AU  - Wang MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120427
PL  - Germany
TA  - J Cancer Res Clin Oncol
JT  - Journal of cancer research and clinical oncology
JID - 7902060
RN  - 0 (DNA Probes)
SB  - IM
CIN - Expert Rev Mol Diagn. 2012 Sep;12(7):679-81. PMID: 23153235
MH  - Adenocarcinoma/diagnosis/genetics
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Aneuploidy
MH  - Bronchi/metabolism/pathology
MH  - Carcinoma, Non-Small-Cell Lung/diagnosis/genetics
MH  - Carcinoma, Squamous Cell/diagnosis/genetics
MH  - Centromere/genetics
MH  - Chromosomes, Human, Pair 3/genetics
MH  - Chromosomes, Human, Pair 7/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - DNA Probes/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Kaplan-Meier Estimate
MH  - Lung Neoplasms/*diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Sensitivity and Specificity
EDAT- 2012/04/28 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/04/28 06:00
PHST- 2012/02/09 00:00 [received]
PHST- 2012/04/10 00:00 [accepted]
PHST- 2012/04/28 06:00 [entrez]
PHST- 2012/04/28 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1007/s00432-012-1232-0 [doi]
PST - ppublish
SO  - J Cancer Res Clin Oncol. 2012 Sep;138(9):1541-9. doi: 10.1007/s00432-012-1232-0. 
      Epub 2012 Apr 27.