PMID- 22539131
OWN - NLM
STAT- MEDLINE
DCOM- 20120904
LR  - 20211021
IS  - 1559-0755 (Electronic)
IS  - 0257-277X (Linking)
VI  - 52
IP  - 3
DP  - 2012 Jun
TI  - A role for anti-CD45RB monoclonal antibody treatment upon dendritic cells.
PG  - 250-7
LID - 10.1007/s12026-012-8336-0 [doi]
AB  - Selective interference with CD45RB isoform by monoclonal antibody 
      (anti-CD45RBmAb) reliably induces donor-specific tolerance. Dendritic cells (DCs) 
      are the most potent antigen-presenting cells that are capable of activating naive 
      T cells. The purposes of the present study were to investigate the roles of 
      anti-CD45RBmAb on the phenotypes and functioning of DCs and to further illustrate 
      the mechanism of anti-CD45RBmAb-inducing immunologic tolerance. DCs from C57BL/6 
      mice were cultured and treated with various doses of anti-CD45RB monoclonal 
      antibody. Cell phenotype, cycle and phagocytic ability were detected by flow 
      cytometry. The production of IL-10 and IL-12 in the supernatants of mature DCs 
      was measured with ELISA. Exosomes (Dex) were recovered from the supernatant of 
      DCs cultured for 6 days in depleted medium, and effects of DCs and Dex on the 
      ability of T-cell proliferation were detected by mixed lymphocyte culture. 
      Anti-CD45RBmAb could inhibit DCs maturation in a dose-dependent manner, and the 
      effects of exosomes (Dex) on DCs enhance or inhibition proliferation of T cells 
      were also in a dose-dependent manner. Anti-CD45RBmAb could profoundly inhibit the 
      maturation and functioning of DCs and generate tolerogenic dendritic cells (tDCs) 
      as well as Dex, suggesting mechanistic contributions to tolerance development 
      from the DCs through interactions with T cells.
FAU - Qi, Hui
AU  - Qi H
AD  - Clinical Medical Research Center, The Second Clinical Medical College, Shenzhen 
      People's Hospital, Ji'nan University, No. 1017 Dongmen North Road, Shenzhen, 
      518020, People's Republic of China.
FAU - Liu, Jin-Peng
AU  - Liu JP
FAU - Deng, Chun-Yan
AU  - Deng CY
FAU - Zhou, Han-Xin
AU  - Zhou HX
FAU - Deng, Shao-Ping
AU  - Deng SP
FAU - Li, Fu-Rong
AU  - Li FR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunol Res
JT  - Immunologic research
JID - 8611087
RN  - 0 (Antibodies, Monoclonal, Murine-Derived)
RN  - 0 (IL10 protein, mouse)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal, Murine-Derived/immunology/*pharmacology
MH  - Cell Communication/drug effects/immunology
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Dose-Response Relationship, Drug
MH  - Immune Tolerance/*drug effects/immunology
MH  - Interleukin-10/immunology
MH  - Interleukin-12/immunology
MH  - Leukocyte Common Antigens/antagonists & inhibitors/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - T-Lymphocytes/*immunology
EDAT- 2012/04/28 06:00
MHDA- 2012/09/05 06:00
CRDT- 2012/04/28 06:00
PHST- 2012/04/28 06:00 [entrez]
PHST- 2012/04/28 06:00 [pubmed]
PHST- 2012/09/05 06:00 [medline]
AID - 10.1007/s12026-012-8336-0 [doi]
PST - ppublish
SO  - Immunol Res. 2012 Jun;52(3):250-7. doi: 10.1007/s12026-012-8336-0.