PMID- 22539132
OWN - NLM
STAT- MEDLINE
DCOM- 20120904
LR  - 20211021
IS  - 1559-0755 (Electronic)
IS  - 0257-277X (Linking)
VI  - 52
IP  - 3
DP  - 2012 Jun
TI  - Prevention and treatment of allergic inflammation by an Fcgamma-Der f2 fusion protein 
      in a murine model of dust mite-induced asthma.
PG  - 276-83
LID - 10.1007/s12026-012-8339-x [doi]
AB  - The immunoglobulin E (IgE) high-affinity receptor FcepsilonRI expressed on mast cells 
      and basophils plays a critical role in triggering allergic disease. The 
      co-aggregation of the FcepsilonRI and FcgammaRIIb receptors is inhibitory to FcepsilonRI 
      signaling and holds great potential for the treatment of IgE-mediated allergies. 
      In China, Dermatophagoides farinae is a common anaphylaxis trigger. Therefore, in 
      this study, the FcgammaRIIb-mediated immunomodulating activity of recombinant Fcgamma-Der 
      f2 fusion protein was tested in a Der f2-allergic murine model. Following the 
      treatment, bronchoalveolar lavage fluid (BALF) was collected to measure the 
      expression of several Th1/Th2-type cytokines (IL-5, TNF-alpha, IL-12p70, IL-4, IL-10, 
      IFN-gamma and IL-18) and histamine, while blood was used to detect the specific IgE 
      and IgG-types anti-Der f2 antibodies, for measurement. In contrast to the 
      saline-treated allergic mice, the levels of Der f2-specific IgE, cytokines and 
      histamine were lowered in the Fcgamma-Der f2-treated allergic mice, in addition to 
      the rare inflammatory cell infiltration in the airways and blood vessels revealed 
      by histopathological examination. The recombinant Fcgamma-Der f2 protein was 
      demonstrated to function as an effective immunotherapeutic agent, suggesting that 
      chimeric human Fcgamma-allergen proteins could be used in the development of 
      antigen-specific immunotherapy for human allergic diseases.
FAU - Lin, Li-hui
AU  - Lin LH
AD  - Department of Laboratory Medicine, Shanghai First People's Hospital, Shanghai 
      Jiao Tong University School of Medicine, 100 Haining Road, Shanghai, 200080, 
      People's Republic of China.
FAU - Zheng, Ping
AU  - Zheng P
FAU - Yuen, John W M
AU  - Yuen JW
FAU - Wang, Juan
AU  - Wang J
FAU - Zhou, Juan
AU  - Zhou J
FAU - Kong, Cun-quan
AU  - Kong CQ
FAU - Peng, Xia
AU  - Peng X
FAU - Li, Jia
AU  - Li J
FAU - Li, Li
AU  - Li L
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Immunol Res
JT  - Immunologic research
JID - 8611087
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Cytokines)
RN  - 0 (Dermatophagoides farinae antigen f 2)
RN  - 0 (Fcgr2b protein, mouse)
RN  - 0 (Receptors, IgG)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Dermatophagoides/genetics/immunology/*pharmacology
MH  - Arthropod Proteins/genetics/immunology/*pharmacology
MH  - Asthma/*drug therapy/immunology/metabolism/pathology
MH  - China
MH  - Cytokines/biosynthesis/immunology
MH  - Dermatophagoides farinae/*immunology
MH  - Disease Models, Animal
MH  - Female
MH  - Gene Expression Regulation/drug effects/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Receptors, IgG
MH  - Recombinant Fusion Proteins/genetics/immunology/pharmacology
MH  - Th1 Cells/immunology/metabolism/pathology
MH  - Th2 Cells/immunology/metabolism/pathology
EDAT- 2012/04/28 06:00
MHDA- 2012/09/05 06:00
CRDT- 2012/04/28 06:00
PHST- 2012/04/28 06:00 [entrez]
PHST- 2012/04/28 06:00 [pubmed]
PHST- 2012/09/05 06:00 [medline]
AID - 10.1007/s12026-012-8339-x [doi]
PST - ppublish
SO  - Immunol Res. 2012 Jun;52(3):276-83. doi: 10.1007/s12026-012-8339-x.