PMID- 22539302
OWN - NLM
STAT- MEDLINE
DCOM- 20120706
LR  - 20181201
IS  - 1521-4141 (Electronic)
IS  - 0014-2980 (Linking)
VI  - 42
IP  - 5
DP  - 2012 May
TI  - IL-6 promotes immune responses in human ulcerative colitis and induces a 
      skin-homing phenotype in the dendritic cells and Tcells they stimulate.
PG  - 1337-53
LID - 10.1002/eji.201142327 [doi]
AB  - Dendritic cells (DCs) control the type and location of immune responses. 
      Ulcerative colitis (UC) is considered a Th2 disease mediated by IL-13 where up to 
      one third of patients can develop extraintestinal manifestations. Colonic 
      biopsies from inflamed and noninflamed areas of UC patients were cultured in 
      vitro and their supernatants were used to condition human blood enriched DCs from 
      healthy controls. Levels of IL-13 in the culture supernatants were below the 
      detection limit in most cases and the cytokine profile suggested a mixed profile 
      rather than a Th2 cytokine profile. IL-6 was the predominant cytokine found in 
      inflamed areas from UC patients and its concentration correlated with the Mayo 
      endoscopic score for severity of disease. DCs conditioned with noninflamed 
      culture supernatants acquired a regulatory phenotype with decreased stimulatory 
      capacity. However, DCs conditioned with inflamed culture supernatants acquired a 
      proinflammatory phenotype with increased expression of the skin-homing chemokine 
      CCR8. These DCs did not have decreased T-cell stimulatory capacity and primed T 
      cells with the skin-homing CLA molecule in an IL-6-dependent mechanism. Our 
      results highlight the role of IL-6 in UC and question the concept of UC as a Th2 
      disease and the relevance of IL-13 in its etiology.
CI  - (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Bernardo, David
AU  - Bernardo D
AD  - Antigen Presentation Research Group, Imperial College London, Northwick Park & St 
      Mark's Campus, Watford Road, Harrow, United Kingdom.
FAU - Vallejo-Diez, Sara
AU  - Vallejo-Diez S
FAU - Mann, Elizabeth R
AU  - Mann ER
FAU - Al-Hassi, Hafid O
AU  - Al-Hassi HO
FAU - Martinez-Abad, Beatriz
AU  - Martinez-Abad B
FAU - Montalvillo, Enrique
AU  - Montalvillo E
FAU - Tee, Cheng T
AU  - Tee CT
FAU - Murugananthan, Aravinth U
AU  - Murugananthan AU
FAU - Nunez, Henar
AU  - Nunez H
FAU - Peake, Simon T C
AU  - Peake ST
FAU - Hart, Ailsa L
AU  - Hart AL
FAU - Fernandez-Salazar, Luis
AU  - Fernandez-Salazar L
FAU - Garrote, Jose A
AU  - Garrote JA
FAU - Arranz, Eduardo
AU  - Arranz E
FAU - Knight, Stella C
AU  - Knight SC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Cytokines)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-13)
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cells, Cultured
MH  - Colitis, Ulcerative/*immunology
MH  - Cytokines/analysis/immunology/metabolism
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Humans
MH  - Inflammation/immunology
MH  - Interleukin-13/immunology
MH  - Interleukin-6/*immunology
MH  - Male
MH  - Middle Aged
MH  - Severity of Illness Index
MH  - Skin/*immunology
MH  - T-Lymphocytes/*immunology
MH  - Th2 Cells/immunology
EDAT- 2012/04/28 06:00
MHDA- 2012/07/07 06:00
CRDT- 2012/04/28 06:00
PHST- 2012/04/28 06:00 [entrez]
PHST- 2012/04/28 06:00 [pubmed]
PHST- 2012/07/07 06:00 [medline]
AID - 10.1002/eji.201142327 [doi]
PST - ppublish
SO  - Eur J Immunol. 2012 May;42(5):1337-53. doi: 10.1002/eji.201142327.