PMID- 22539814
OWN - NLM
STAT- MEDLINE
DCOM- 20121001
LR  - 20211021
IS  - 1537-6613 (Electronic)
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 206
IP  - 1
DP  - 2012 Jul 1
TI  - Penicillin-binding protein of Ehrlichia chaffeensis: cytokine induction through 
      MyD88-dependent pathway.
PG  - 110-6
LID - 10.1093/infdis/jis313 [doi]
AB  - BACKGROUND: Human monocytic ehrlichiosis is one of the most prevalent tick-borne 
      zoonoses caused by infection with Ehrlichia chaffeensis. Although E. chaffeensis 
      lacks entire lipopolysaccharide and most peptidoglycan biosynthesis genes, it 
      induces inflammatory cytokines and chemokines. Ehrlichia chaffeensis components 
      that induce inflammation and the responsive host cell pathway are not known. 
      METHODS: Expression of penicillin-binding protein (PBP) in E. chaffeensis was 
      analyzed by reverse-transcription polymerase chain reaction and Bocillin FL 
      binding assay. Next, recombinant PBP, which was high-pressure liquid 
      chromatography purified, and native PBP of E. chaffeensis were investigated for 
      their ability to induce proinflammatory cytokines in the human monocytic leukemia 
      cell line THP-1 and bone marrow-derived macrophages (BMDMs) from wild-type and 
      MyD88 knockout mice. RESULTS: Expression of PBP by E. chaffeensis was upregulated 
      during its intracellular life cycle. PBP induced interleukin 8 or CXCL2, tumor 
      necrosis factor alpha, interleukin 1beta, and interleukin 10 in THP-1 cells and BMDMs. 
      Cytokine induction by PBP was MyD88-dependent. Removal of PBP from E. chaffeensis 
      lysate using penicillin affinity column and a complementation assay confirmed 
      cytokine-inducing activity of native PBP. CONCLUSIONS: The cytokine-inducing 
      activity by E. chaffeensis PBP provides novel insights into pathogen-associated 
      molecular patterns and pathogenesis of E. chaffeensis infection.
FAU - Rahman, Mohd Akhlakur
AU  - Rahman MA
AD  - Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio 
      State University, Columbus, OH 43210-1093, USA.
FAU - Cheng, Zhihui
AU  - Cheng Z
FAU - Matsuo, Junji
AU  - Matsuo J
FAU - Rikihisa, Yasuko
AU  - Rikihisa Y
LA  - eng
GR  - R01 AI047885/AI/NIAID NIH HHS/United States
GR  - R01AI47885/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120426
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-8)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Penicillin-Binding Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Chemokine CXCL2/biosynthesis/genetics/immunology
MH  - Cytokines/*biosynthesis/genetics/immunology
MH  - Ehrlichia chaffeensis/genetics/immunology/*metabolism
MH  - Ehrlichiosis/genetics/metabolism
MH  - Humans
MH  - Interleukin-10/biosynthesis/genetics/immunology
MH  - Interleukin-1beta/biosynthesis/genetics/immunology
MH  - Interleukin-8/biosynthesis/genetics/immunology
MH  - Macrophages/immunology/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Monocytes/metabolism
MH  - Myeloid Differentiation Factor 88/genetics/immunology/*metabolism
MH  - Penicillin-Binding Proteins/genetics/immunology/*metabolism
MH  - Tumor Necrosis Factor-alpha/biosynthesis/genetics/immunology
PMC - PMC3415931
EDAT- 2012/04/28 06:00
MHDA- 2012/10/02 06:00
PMCR- 2013/07/01
CRDT- 2012/04/28 06:00
PHST- 2012/04/28 06:00 [entrez]
PHST- 2012/04/28 06:00 [pubmed]
PHST- 2012/10/02 06:00 [medline]
PHST- 2013/07/01 00:00 [pmc-release]
AID - jis313 [pii]
AID - 10.1093/infdis/jis313 [doi]
PST - ppublish
SO  - J Infect Dis. 2012 Jul 1;206(1):110-6. doi: 10.1093/infdis/jis313. Epub 2012 Apr 
      26.