PMID- 22541162
OWN - NLM
STAT- MEDLINE
DCOM- 20130823
LR  - 20181201
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 159
IP  - 3-4
DP  - 2012 Oct 12
TI  - Avirulent K88 (F4)+ Escherichia coli strains constructed to express modified 
      enterotoxins protect young piglets from challenge with a virulent enterotoxigenic 
      Escherichia coli strain that expresses the same adhesion and enterotoxins.
PG  - 337-42
LID - 10.1016/j.vetmic.2012.03.046 [doi]
AB  - Virulence of enterotoxigenic Escherichia coli (ETEC) is associated with fimbrial 
      adhesins and enterotoxins such as heat-labile (LT) and/or heat-stable (ST) 
      enterotoxins. Previous studies using a cell culture model suggest that exclusion 
      of ETEC from attachment to epithelial cells requires expression of both an 
      adhesin such as K88 (F4) fimbriae, and LT. To test the ability of non-pathogenic 
      E. coli constructs to exclude virulent ETEC sufficiently to prevent clinical 
      disease, we utilized a piglet ETEC challenge model. Thirty-nine 5-day-old piglets 
      were inoculated with a placebo (control), or with either of the three K88(+)E. 
      coli strains isogenic with regard to modified LT expression: 8017 (pBR322 plasmid 
      vector control), non-toxigenic mutant 8221 (LT(R192G)) in pBR322, or 8488, with 
      the LT gene fused to the STb gene in pBR322 (LT(R192G)-STb). Piglets were 
      challenged with virulent ETEC Strain 3030-2 (K88(+)/LT/STb) 24h post-inoculation. 
      K88ac receptor-positive piglets in the control group developed diarrhea and 
      became dehydrated 12-24h post-challenge. Piglets inoculated with 8221 or 8488 did 
      not exhibit clinical signs of ETEC disease; most piglets inoculated with 8017 
      showed diarrhea. Control pigs exhibited significant weight loss, increased blood 
      total protein, and higher numbers of colony-forming units of 3030-2 E. coli in 
      washed ileum and jejunum than treated pigs. This study shows for the first time 
      that pre-inoculation with an avirulent strain expressing adhesive fimbriae and a 
      non-toxic form of LT provides significant short term protection from challenge 
      with a virulent ETEC strain that expresses the same fimbrial adhesion and 
      enterotoxin.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Santiago-Mateo, Kristina
AU  - Santiago-Mateo K
AD  - Center for Infectious Disease Research and Vaccine Development, Department of 
      Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 
      57007-1396, USA.
FAU - Zhao, Mojun
AU  - Zhao M
FAU - Lin, Jun
AU  - Lin J
FAU - Zhang, Weiping
AU  - Zhang W
FAU - Francis, David H
AU  - Francis DH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120406
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Adhesins, Bacterial)
RN  - 0 (Bacterial Vaccines)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
SB  - IM
MH  - Adhesins, Bacterial/genetics/immunology
MH  - Animals
MH  - Bacterial Vaccines/*administration & dosage/immunology
MH  - Diarrhea/immunology/prevention & control/veterinary
MH  - Enterotoxigenic Escherichia coli/classification/pathogenicity/*physiology
MH  - Enterotoxins/genetics/immunology
MH  - Escherichia coli/pathogenicity/*physiology
MH  - Escherichia coli Infections/immunology/prevention & control/*veterinary
MH  - Escherichia coli Proteins/genetics/immunology/toxicity
MH  - Fimbriae, Bacterial/genetics/immunology
MH  - Swine
MH  - Swine Diseases/*immunology/*prevention & control
MH  - Virulence
EDAT- 2012/05/01 06:00
MHDA- 2013/08/24 06:00
CRDT- 2012/05/01 06:00
PHST- 2012/01/28 00:00 [received]
PHST- 2012/03/30 00:00 [revised]
PHST- 2012/03/31 00:00 [accepted]
PHST- 2012/05/01 06:00 [entrez]
PHST- 2012/05/01 06:00 [pubmed]
PHST- 2013/08/24 06:00 [medline]
AID - S0378-1135(12)00214-3 [pii]
AID - 10.1016/j.vetmic.2012.03.046 [doi]
PST - ppublish
SO  - Vet Microbiol. 2012 Oct 12;159(3-4):337-42. doi: 10.1016/j.vetmic.2012.03.046. 
      Epub 2012 Apr 6.