PMID- 2254196
OWN - NLM
STAT- MEDLINE
DCOM- 19910124
LR  - 20190510
IS  - 0021-8812 (Print)
IS  - 0021-8812 (Linking)
VI  - 68
IP  - 10
DP  - 1990 Oct
TI  - Growth hormone release after N-methyl-D,L-aspartate in sheep: dose response and 
      effect of an opioid antagonist.
PG  - 3198-203
AB  - The objectives of our experiments were 1) to determine the effect of 
      N-methyl-D,L-aspartate (NMA), an agonist of the neuroexcitatory amino acids 
      aspartate and glutamate, on growth hormone (GH) release in ovariectomized ewes, 
      and 2) to determine the effect of naloxone, an opioid antagonist, on the GH 
      response to NMA. Jugular blood was collected via venipuncture at 12-min intervals 
      for 2 h before and 2 h after i.v. injection of NMA. In Exp. 1, ewes received 
      either 0, 6, 12 or 24 mg NMA/kg BW dissolved in .9% saline solution (n = 4 per 
      treatment). Growth hormone concentrations were similar (P greater than .1) 
      between groups prior to injection (9.8 +/- .7 ng/ml; mean +/- SEM) and were 
      unaffected (P greater than .1) by saline treatment. In contrast, 6, 12 or 24 mg 
      NMA/kg BW increased mean GH concentration by 210% (P less than .04), 273% (P less 
      than .02) and 234% (P less than .02), respectively. In Exp. 2, ewes received NMA 
      (6 mg/kg BW) 5 min after either saline (n = 4) or naloxone (1 mg/kg BW; n = 4) 
      pretreatment. Serum GH concentrations averaged 7.0 +/- 1.1 ng/ml before 
      pretreatment and increased similarly (238%; P greater than .1) in both groups 
      following NMA. In summary, NMA increased GH concentrations in ovariectomized ewes 
      by some mechanism that does not involve opioid receptors that are antagonized by 
      naloxone.
FAU - Estienne, M J
AU  - Estienne MJ
AD  - Dept. of Anim. Sci., University of Kentucky, Lexington 40546-0215.
FAU - Schillo, K K
AU  - Schillo KK
FAU - Green, M A
AU  - Green MA
FAU - Hileman, S M
AU  - Hileman SM
LA  - eng
GR  - HD 23345-01/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 36B82AMQ7N (Naloxone)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 9002-72-6 (Growth Hormone)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Female
MH  - Growth Hormone/drug effects/*metabolism
MH  - N-Methylaspartate/*pharmacology
MH  - Naloxone/pharmacology
MH  - Ovariectomy/veterinary
MH  - Sheep/*metabolism
MH  - Sodium Chloride/pharmacology
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
AID - 10.2527/1990.68103198x [doi]
PST - ppublish
SO  - J Anim Sci. 1990 Oct;68(10):3198-203. doi: 10.2527/1990.68103198x.