PMID- 22542910
OWN - NLM
STAT- MEDLINE
DCOM- 20121012
LR  - 20121115
IS  - 1872-7603 (Electronic)
IS  - 0165-0378 (Linking)
VI  - 94
IP  - 2
DP  - 2012 Jun
TI  - Trophoblast debris modulates the expression of immune proteins in macrophages: a 
      key to maternal tolerance of the fetal allograft?
PG  - 131-41
LID - 10.1016/j.jri.2012.03.488 [doi]
AB  - Interactions between maternal immune cells and the placenta are of substantial 
      interest since diseases of pregnancy, such as recurrent miscarriage, villitis of 
      unknown etiology and preeclampsia may arise due to inadequate adaptation of the 
      maternal immune system. During normal pregnancy trophoblast debris is shed from 
      the placenta into the maternal blood in large quantities. This trophoblast debris 
      is then rapidly cleared from the maternal circulation. In this study, we exposed 
      trophoblast debris generated from an in vitro placental explant model to 
      peripheral blood-derived macrophages and quantified a variety of molecules that 
      are important in immune responses by ELISA or flow cytometry. Phagocytosis of 
      trophoblast debris resulted in reduced cell-surface expression of MHC-II 
      molecules, the costimulatory molecules (CD80, CD86, CD40 and B7H3), monocyte 
      chemoattractant protein-1 (MCP-1), inter-cellular adhesion molecule 1 (ICAM-1) 
      and IL-8 receptors in macrophages while the expression of programmed death-1 
      ligand 1 (PD-L1) was upregulated. In addition, phagocytosis of trophoblast debris 
      induced the secretion of the anti-inflammatory cytokines IL-10, IL6 and IL1Ra and 
      decreased the secretion of pro-inflammatory cytokines IL-1beta, IL12p70 and IL-8 by 
      macrophages. Phagocytosis of trophoblast debris also increased macrophage 
      expression of the immunosuppressive enzyme indoleamine 2,3-dioxygenase (IDO). We 
      have shown that phagocytosis of trophoblast debris from normal placentae alters 
      the phenotype of macrophages such that they are likely to deviate maternal immune 
      responses towards tolerance and away from inflammation. This may be one of the 
      mechanisms that allow the human fetal allograft to survive in direct contact with 
      the maternal immune system.
CI  - Copyright (c) 2012 Elsevier Ireland Ltd. All rights reserved.
FAU - Abumaree, M H
AU  - Abumaree MH
AD  - King Saud Bin Abdulaziz University for Health Sciences, King Abdullah 
      International Medical Research Center, King Abdulaziz Medical City, National 
      Guard Health Affairs, P.O. Box 22490, Riyadh 11426, Mail Code 1515, Saudi Arabia. 
      abumareem@ksau-hs.edu.sa
FAU - Chamley, L W
AU  - Chamley LW
FAU - Badri, M
AU  - Badri M
FAU - El-Muzaini, M F
AU  - El-Muzaini MF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120427
PL  - Ireland
TA  - J Reprod Immunol
JT  - Journal of reproductive immunology
JID - 8001906
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase)
RN  - 0 (Inflammation Mediators)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Antigens, CD/genetics/metabolism
MH  - Cells, Cultured
MH  - Cellular Structures/cytology/*immunology/metabolism
MH  - Cytokines/genetics/metabolism
MH  - Female
MH  - Fetus/*immunology
MH  - Gene Expression Regulation, Developmental/immunology
MH  - Histocompatibility Antigens Class II/genetics/metabolism
MH  - Humans
MH  - Immune Tolerance
MH  - Indoleamine-Pyrrole 2,3,-Dioxygenase/genetics/metabolism
MH  - Inflammation Mediators/metabolism
MH  - Intercellular Adhesion Molecule-1/genetics/metabolism
MH  - Macrophages/*immunology
MH  - Placental Circulation/immunology
MH  - Pregnancy
MH  - *Transplantation Tolerance
MH  - Transplantation, Homologous/*immunology
MH  - Trophoblasts/cytology/*immunology/metabolism
EDAT- 2012/05/01 06:00
MHDA- 2012/10/13 06:00
CRDT- 2012/05/01 06:00
PHST- 2011/11/04 00:00 [received]
PHST- 2012/03/19 00:00 [revised]
PHST- 2012/03/26 00:00 [accepted]
PHST- 2012/05/01 06:00 [entrez]
PHST- 2012/05/01 06:00 [pubmed]
PHST- 2012/10/13 06:00 [medline]
AID - S0165-0378(12)00556-6 [pii]
AID - 10.1016/j.jri.2012.03.488 [doi]
PST - ppublish
SO  - J Reprod Immunol. 2012 Jun;94(2):131-41. doi: 10.1016/j.jri.2012.03.488. Epub 
      2012 Apr 27.