PMID- 22543102
OWN - NLM
STAT- MEDLINE
DCOM- 20121113
LR  - 20131121
IS  - 1872-9754 (Electronic)
IS  - 0197-0186 (Linking)
VI  - 61
IP  - 1
DP  - 2012 Jul
TI  - The expression of NR2B subunit of NMDA receptor in the suprachiasmatic nucleus of 
      Wistar rats and its role in glutamate-induced CREB and ERK1/2 phosphorylation.
PG  - 43-7
LID - 10.1016/j.neuint.2012.04.016 [doi]
AB  - Most behavioral and physiological processes in living organisms exhibit periodic 
      circadian rhythmicity. In mammals, these rhythms are coordinated by the circadian 
      clock located in the suprachiasmatic nucleus (SCN) of the hypothalamus. In order 
      to precisely synchronize free-running circadian oscillations to the 24h solar 
      cycle, signals from the external environment, primarily the light/dark cycle, 
      must reach the circadian clock within the SCN. A light pulse elevates 
      intracellular Ca(2+) levels, and activates signaling cascades, leading to 
      transcriptional activation of the clock genes mPer1 and mPer2 via phosphorylation 
      of extracellular-signal-regulated kinases 1/2 (ERK1/2) and cyclic AMP-responsive 
      element binding protein (CREB). Glutamate is the primary excitatory transmitter 
      in retinal terminals in the SCN, and NMDA receptors (NMDAR) are the principal 
      glutamate receptors that mediate the effect of light on resetting the circadian 
      clock. Here we show the circadian rhythm in mRNA expression and protein level of 
      the NMDAR 2B subunit (NR2B) in the SCN, with a peak at night. Also, we 
      demonstrate ifenprodil inhibition of glutamate-induced phosphorylation of CREB 
      (pCREB) and ERK1/2 (pERK1/2), and support thus the evidence for NR2B role in 
      activation of signaling cascade involved in photic resetting of the circadian 
      clock.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Bendova, Zdenka
AU  - Bendova Z
AD  - Institute of Physiology, Academy of Sciences of the Czech Republic v.v.i., 
      Prague, Czech Republic. zdenka.bendova@natur.cuni.cz
FAU - Sladek, Martin
AU  - Sladek M
FAU - Svobodova, Irena
AU  - Svobodova I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120421
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (Piperidines)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - R8OE3P6O5S (ifenprodil)
SB  - IM
MH  - Animals
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Glutamic Acid/*metabolism
MH  - In Situ Hybridization
MH  - *MAP Kinase Signaling System
MH  - Male
MH  - Phosphorylation
MH  - Piperidines/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Suprachiasmatic Nucleus/drug effects/*metabolism
EDAT- 2012/05/01 06:00
MHDA- 2012/11/14 06:00
CRDT- 2012/05/01 06:00
PHST- 2012/02/03 00:00 [received]
PHST- 2012/04/13 00:00 [revised]
PHST- 2012/04/15 00:00 [accepted]
PHST- 2012/05/01 06:00 [entrez]
PHST- 2012/05/01 06:00 [pubmed]
PHST- 2012/11/14 06:00 [medline]
AID - S0197-0186(12)00147-7 [pii]
AID - 10.1016/j.neuint.2012.04.016 [doi]
PST - ppublish
SO  - Neurochem Int. 2012 Jul;61(1):43-7. doi: 10.1016/j.neuint.2012.04.016. Epub 2012 
      Apr 21.