PMID- 22544527
OWN - NLM
STAT- MEDLINE
DCOM- 20120713
LR  - 20220330
IS  - 1529-0131 (Electronic)
IS  - 0004-3591 (Linking)
VI  - 64
IP  - 5
DP  - 2012 May
TI  - Promotion of bone repair by implantation of cryopreserved bone marrow-derived 
      mononuclear cells in a rabbit model of steroid-associated osteonecrosis.
PG  - 1562-71
LID - 10.1002/art.34525 [doi]
AB  - OBJECTIVE: Cytotherapy is an insufficient method for promoting bone repair in 
      steroid-associated osteonecrosis (SAON), and this has been attributed to 
      impairment of the bioactivity of bone marrow-derived stem cells (BMSCs) after 
      pulsed administration of steroids. Cryopreserved autologous bone marrow-derived 
      mononuclear cells (BMMNCs), which contain BMSCs, might maintain their bioactivity 
      in vitro. This study sought to investigate the effects of cryopreserved BMMNCs, 
      before steroid administration, on the enhancement of bone repair in an 
      established rabbit model of SAON. METHODS: For in vitro study, bone marrow was 
      harvested 4 weeks before SAON induction from the iliac crests of rabbits (n = 10) 
      to isolate fresh BMMNCs, and the BMMNCs were then cryopreserved for 8 weeks. Both 
      the fresh and the cryopreserved BMMNCs were evaluated for their bioactivity and 
      osteogenic differentiation capacity. In addition, BMMNCs were isolated 2 weeks 
      after SAON induction and subjected to the same evaluations. For in vivo study, 
      cryopreserved BMMNCs were implanted into the bone tunnel during core 
      decompression of the femur (n = 12 rabbits) after the induction of SAON, and 
      tissue regeneration was evaluated by micro-computed tomography and histologic 
      analyses at 12 weeks postoperation. RESULTS: In vitro, there were no significant 
      differences in the bioactivity or ability to undergo osteogenic differentiation 
      between fresh BMMNCs and cryopreserved BMMNCs, but after SAON induction, both 
      features were decreased significantly. In vivo, the bone mineral density, ratio 
      of bone volume to total volume of bone, and volume and diameter of 
      neovascularization within the bone tunnel were significantly higher in the 
      BMMNC-treated group compared to the nontreated control group at 12 weeks 
      postoperation. CONCLUSION: Cryopreserved BMMNCs maintained their bioactivity and 
      promoted bone regeneration and neovascularization within the bone tunnel after 
      core decompression in this rabbit model of SAON.
CI  - Copyright (c) 2012 by the American College of Rheumatology.
FAU - Xie, Xin-Hui
AU  - Xie XH
AD  - The Chinese University of Hong Kong, Hong Kong, China.
FAU - Wang, Xin-Luan
AU  - Wang XL
FAU - He, Yi-Xin
AU  - He YX
FAU - Liu, Zhong
AU  - Liu Z
FAU - Sheng, Hui
AU  - Sheng H
FAU - Zhang, Ge
AU  - Zhang G
FAU - Qin, Ling
AU  - Qin L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Glucocorticoids)
RN  - X4W7ZR7023 (Methylprednisolone)
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/*methods
MH  - Bone Regeneration/*physiology
MH  - *Cryopreservation
MH  - Disease Models, Animal
MH  - Femur Head/drug effects/pathology
MH  - Femur Head Necrosis/chemically induced/pathology/*therapy
MH  - Glucocorticoids/toxicity
MH  - Male
MH  - Methylprednisolone/toxicity
MH  - Monocytes/*transplantation
MH  - Osteonecrosis/*therapy
MH  - Rabbits
EDAT- 2012/05/01 06:00
MHDA- 2012/07/14 06:00
CRDT- 2012/05/01 06:00
PHST- 2012/05/01 06:00 [entrez]
PHST- 2012/05/01 06:00 [pubmed]
PHST- 2012/07/14 06:00 [medline]
AID - 10.1002/art.34525 [doi]
PST - ppublish
SO  - Arthritis Rheum. 2012 May;64(5):1562-71. doi: 10.1002/art.34525.