PMID- 22546502
OWN - NLM
STAT- MEDLINE
DCOM- 20130215
LR  - 20220317
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Linking)
VI  - 146
IP  - 1-2
DP  - 2012 Aug 30
TI  - Retinoic acid receptor beta deficiency reduces splenic dendritic cell population in 
      a conditional mouse line.
PG  - 15-24
LID - 10.1016/j.imlet.2012.04.006 [doi]
AB  - Different studies have shown that retinoids and their receptors [retinoic acid 
      receptors (RARs) and retinoid X receptors (RXRs)] have crucial effects on the 
      differentiation and function of myeloid cells such as Dendritic cells (DCs) and 
      the development of lymphoid tissue. However, the relationship between RARbeta 
      expression and DCs has not been previously studied in vivo. This work examined 
      the effect of decreased RARbeta expression on the number (and probably on 
      differentiation) of splenic DCs and the structure of spleen using a conditional 
      mouse that partially ablates floxed RARbeta gene (RARbeta(L-/L-) mice). Our results 
      showed that RARbeta is expressed mainly in cells of the splenic White Pulp (WP) zone 
      of Wild type mice. As expected, low levels of RARbeta expression were detected in 
      the spleen of RARbeta(L-/L-) conditional mice. These results were consistent with a 
      decrease in the population of splenic CD11c(+)MHC-II(+) cells. Histopathological 
      analyses of conditional mice spleen indicated defects in cell organization and 
      structure. The expression of Toll-like receptor 2 was also down-regulated in the 
      spleen of these mice. These results suggest that RARbeta is involved in splenic cell 
      organization as well as in the maintenance of splenic DCs population, indicating 
      that RARbeta expression is important in homeostasis of immune system components.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Serafin-Higuera, Nicolas
AU  - Serafin-Higuera N
AD  - Dept. of Genetics and Molecular Biology, CINVESTAV-IPN, 07360, Mexico, DF, 
      Mexico.
FAU - Hernandez-Sanchez, Javier
AU  - Hernandez-Sanchez J
FAU - Ocadiz-Delgado, Rodolfo
AU  - Ocadiz-Delgado R
FAU - Vazquez-Hernandez, Juan
AU  - Vazquez-Hernandez J
FAU - Albino-Sanchez, Martha Estela
AU  - Albino-Sanchez ME
FAU - Hernandez-Pando, Rogelio
AU  - Hernandez-Pando R
FAU - Gariglio, Patricio
AU  - Gariglio P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120421
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (CD11 Antigens)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (retinoic acid receptor beta)
SB  - IM
MH  - Animals
MH  - CD11 Antigens/genetics/immunology
MH  - Cell Lineage/genetics/*immunology
MH  - Dendritic Cells/*immunology/metabolism/pathology
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression/*immunology
MH  - Homeostasis/genetics/immunology
MH  - Immunity/genetics
MH  - Immunohistochemistry
MH  - Lymphocyte Count
MH  - Mice
MH  - Mice, Knockout
MH  - Receptors, Retinoic Acid/deficiency/genetics/*immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spleen/immunology/metabolism/*pathology
MH  - Toll-Like Receptor 2/genetics/immunology
EDAT- 2012/05/02 06:00
MHDA- 2013/02/16 06:00
CRDT- 2012/05/02 06:00
PHST- 2011/11/18 00:00 [received]
PHST- 2012/03/27 00:00 [revised]
PHST- 2012/04/13 00:00 [accepted]
PHST- 2012/05/02 06:00 [entrez]
PHST- 2012/05/02 06:00 [pubmed]
PHST- 2013/02/16 06:00 [medline]
AID - S0165-2478(12)00109-5 [pii]
AID - 10.1016/j.imlet.2012.04.006 [doi]
PST - ppublish
SO  - Immunol Lett. 2012 Aug 30;146(1-2):15-24. doi: 10.1016/j.imlet.2012.04.006. Epub 
      2012 Apr 21.