PMID- 22548193 OWN - NLM STAT- MEDLINE DCOM- 20120802 LR - 20230429 IS - 1687-5443 (Electronic) IS - 2090-5904 (Print) IS - 1687-5443 (Linking) VI - 2012 DP - 2012 TI - Divergent roles of p75NTR and Trk receptors in BDNF's effects on dendritic spine density and morphology. PG - 578057 LID - 10.1155/2012/578057 [doi] LID - 578057 AB - Activation of TrkB receptors by brain-derived neurotrophic factor (BDNF) followed by MAPK/ERK signaling increases dendritic spine density and the proportion of mature spines in hippocampal CA1 pyramidal neurons. Considering the opposing actions of p75(NTR) and Trk receptors in several BDNF actions on CNS neurons, we tested whether these receptors also have divergent actions on dendritic spine density and morphology. A function-blocking anti-p75(NTR) antibody (REX) did not affect spine density by itself but it prevented BDNF's effect on spine density. Intriguingly, REX by itself increased the proportion of immature spines and prevented BDNF's effect on spine morphology. In contrast, the Trk receptor inhibitor k-252a increased spine density by itself, and prevented BDNF from further increasing spine density. However, most of the spines in k-252a-treated slices were of the immature type. These effects of k-252a on spine density and morphology required neuronal activity because they were prevented by TTX. These divergent BDNF actions on spine density and morphology are reminiscent of opposing functional signaling by p75(NTR) and Trk receptors and reveal an unexpected level of complexity in the consequences of BDNF signaling on dendritic morphology. FAU - Chapleau, Christopher A AU - Chapleau CA AD - Department of Neurobiology, SHEL-1002, Civitan International Research Center, The University of Alabama at Birmingham, 1825 University Boulevard, Birmingham, AL 35294-2182, USA. FAU - Pozzo-Miller, Lucas AU - Pozzo-Miller L LA - eng GR - P30 NS047466/NS/NINDS NIH HHS/United States GR - P30 NS057098/NS/NINDS NIH HHS/United States GR - P30 HD038985/HD/NICHD NIH HHS/United States GR - NS057780/NS/NINDS NIH HHS/United States GR - R01 NS040593/NS/NINDS NIH HHS/United States GR - NS065027/NS/NINDS NIH HHS/United States GR - R01 NS065027/NS/NINDS NIH HHS/United States GR - R21 NS057780/NS/NINDS NIH HHS/United States GR - NS40593/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20120327 PL - United States TA - Neural Plast JT - Neural plasticity JID - 100883417 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Nerve Growth Factors) RN - 0 (Nerve Tissue Proteins) RN - 0 (Receptors, Growth Factor) RN - 0 (Receptors, Nerve Growth Factor) RN - 136958-07-1 (Ngfr protein, rat) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*metabolism/physiology MH - Dendritic Spines/*metabolism/*ultrastructure MH - Hippocampus/metabolism MH - Nerve Growth Factors/metabolism/pharmacology MH - Nerve Tissue Proteins MH - Neurons/metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Receptor, trkB/*metabolism/physiology MH - Receptors, Growth Factor MH - Receptors, Nerve Growth Factor/antagonists & inhibitors/*metabolism/physiology MH - Signal Transduction/physiology PMC - PMC3323862 EDAT- 2012/05/02 06:00 MHDA- 2012/08/03 06:00 PMCR- 2012/03/27 CRDT- 2012/05/02 06:00 PHST- 2011/11/29 00:00 [received] PHST- 2012/01/13 00:00 [revised] PHST- 2012/01/13 00:00 [accepted] PHST- 2012/05/02 06:00 [entrez] PHST- 2012/05/02 06:00 [pubmed] PHST- 2012/08/03 06:00 [medline] PHST- 2012/03/27 00:00 [pmc-release] AID - 10.1155/2012/578057 [doi] PST - ppublish SO - Neural Plast. 2012;2012:578057. doi: 10.1155/2012/578057. Epub 2012 Mar 27.