PMID- 22551928
OWN - NLM
STAT- MEDLINE
DCOM- 20130517
LR  - 20211021
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Print)
IS  - 0171-2985 (Linking)
VI  - 218
IP  - 1
DP  - 2013 Jan
TI  - Adhesion to substrates induces dendritic cell endothelization and decreases 
      immunological response.
PG  - 64-75
LID - S0171-2985(12)00039-3 [pii]
LID - 10.1016/j.imbio.2012.02.003 [doi]
AB  - Dendritic cells (DCs) are antigen presenting cells capable of inducing specific 
      immune responses against microbial infections, transplant antigens, or tumors. 
      DCs have been shown to possess a high plasticity showing different phenotypes in 
      response to their microenvironment. For example, tumor-associated DCs can acquire 
      an angiogenic phenotype thus promoting tumor growth. Further, DCs cultured in 
      vitro under different conditions are able to upregulate the expression of 
      endothelial markers and to express angiogenic factors. Indeed, it has been shown 
      that soluble factors such as VEGF of PGE-2, that are present in the 
      microenvironment of several tumors, affect the biology of these cells. We 
      hypothesize that in addition to soluble factors the adhesion to different 
      substrates will also define the phenotype and function of DCs. Herewith we 
      demonstrate that murine myeloid(m) DCs upregulate endothelial markers such as 
      VE-Cadherin, and to a lesser extent TIE-2, and decrease their immune capabilities 
      when cultured on solid surfaces as compared with the same cells cultured on 
      ultra-low binding (ULB) surfaces. On the other hand, the expression of angiogenic 
      molecules at the level of RNA was not different among these cultures. In order to 
      further investigate this phenomenon we used the murine ID8 model of ovarian 
      cancer which can generate solid tumors when cancer cells are injected 
      subcutaneously or a malignant ascites when they are injected intraperitoneally. 
      This model gave us the unique opportunity to investigate DCs in suspension or 
      attached to solid surfaces under the influence of the same tumor cells. We were 
      able to determine that DCs present in solid tumors showed higher levels of 
      expression of endothelial markers and angiogenic molecules but were not able to 
      respond to inflammatory stimuli at the same extent as DCs recovered from ascites. 
      Moreover, mDCs cultured on ULB surfaces in the presence of tumor factors do not 
      expressed endothelial markers. Taking into account all these data we consider 
      that tumor factors might be responsible for inducing angiogenic properties in 
      DCs, but that in some settings the expression of endothelial markers such as 
      VE-Cadherin and TIE-2 might be a function of attachment to solid surfaces and 
      independent of the angiogenic properties of these cells.
CI  - Copyright (c) 2012 Elsevier GmbH. All rights reserved.
FAU - Osterbur, Jacob
AU  - Osterbur J
AD  - Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio 
      University, Athens, OH 45701, USA.
FAU - Sprague, Leslee
AU  - Sprague L
FAU - Muccioli, Maria
AU  - Muccioli M
FAU - Pate, Michelle
AU  - Pate M
FAU - Mansfield, Kristen
AU  - Mansfield K
FAU - McGinty, John
AU  - McGinty J
FAU - Li, Yihan
AU  - Li Y
FAU - Li, Yandi
AU  - Li Y
FAU - Shirure, Venktesh
AU  - Shirure V
FAU - Courreges, Maria Cecilia
AU  - Courreges MC
FAU - Benencia, Fabian
AU  - Benencia F
LA  - eng
GR  - R15 CA137499/CA/NCI NIH HHS/United States
GR  - R15 CA137499-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120227
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Antigens, CD)
RN  - 0 (Cadherins)
RN  - 0 (Interleukin-6)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (cadherin 5)
RN  - 0 (vascular endothelial growth factor A, mouse)
RN  - EC 2.7.10.1 (Receptor, TIE-2)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/metabolism
MH  - Cadherins/genetics/metabolism
MH  - Cell Adhesion/immunology
MH  - Cell Differentiation
MH  - Cell Line, Tumor
MH  - Dendritic Cells/*immunology
MH  - Endothelium/immunology/*metabolism
MH  - Female
MH  - Humans
MH  - Interleukin-6/genetics/metabolism
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Ovarian Neoplasms/*immunology
MH  - Receptor, TIE-2/genetics/metabolism
MH  - Tumor Microenvironment
MH  - Up-Regulation
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
PMC - PMC3413747
MID - NIHMS360485
EDAT- 2012/05/04 06:00
MHDA- 2013/05/18 06:00
PMCR- 2014/01/01
CRDT- 2012/05/04 06:00
PHST- 2011/07/11 00:00 [received]
PHST- 2012/02/01 00:00 [revised]
PHST- 2012/02/04 00:00 [accepted]
PHST- 2012/05/04 06:00 [entrez]
PHST- 2012/05/04 06:00 [pubmed]
PHST- 2013/05/18 06:00 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - S0171-2985(12)00039-3 [pii]
AID - 10.1016/j.imbio.2012.02.003 [doi]
PST - ppublish
SO  - Immunobiology. 2013 Jan;218(1):64-75. doi: 10.1016/j.imbio.2012.02.003. Epub 2012 
      Feb 27.