PMID- 22553154
OWN - NLM
STAT- MEDLINE
DCOM- 20121019
LR  - 20220331
IS  - 1549-4918 (Electronic)
IS  - 1066-5099 (Linking)
VI  - 30
IP  - 7
DP  - 2012 Jul
TI  - Analysis of tissues following mesenchymal stromal cell therapy in humans 
      indicates limited long-term engraftment and no ectopic tissue formation.
PG  - 1575-8
LID - 10.1002/stem.1118 [doi]
AB  - Mesenchymal stromal cells (MSCs) are explored as a novel treatment for a variety 
      of medical conditions. Their fate after infusion is unclear, and long-term safety 
      regarding malignant transformation and ectopic tissue formation has not been 
      addressed in patients. We examined autopsy material from 18 patients who had 
      received human leukocyte antigen (HLA)-mismatched MSCs, and 108 tissue samples 
      from 15 patients were examined by PCR. No signs of ectopic tissue formation or 
      malignant tumors of MSC-donor origin were found on macroscopic or histological 
      examination. MSC donor DNA was detected in one or several tissues including 
      lungs, lymph nodes, and intestine in eight patients at levels from 1/100 to 
      <1/1,000. Detection of MSC donor DNA was negatively correlated with time from 
      infusion to sample collection, as DNA was detected from nine of 13 MSC infusions 
      given within 50 days before sampling but from only two of eight infusions given 
      earlier. There was no correlation between MSC engraftment and treatment response. 
      We conclude that MSCs appear to mediate their function through a "hit and run" 
      mechanism. The lack of sustained engraftment limits the long-term risks of MSC 
      therapy.
CI  - Copyright (c) 2012 AlphaMed Press.
FAU - von Bahr, L
AU  - von Bahr L
AD  - Division of Clinical Immunology and Transfusion Medicine, Department of 
      Laboratory Medicine; Hematology Center, Karolinska University Hospital, 
      Stockholm, Sweden. Lena.von.bahr@ki.se
FAU - Batsis, I
AU  - Batsis I
FAU - Moll, G
AU  - Moll G
FAU - Hagg, M
AU  - Hagg M
FAU - Szakos, A
AU  - Szakos A
FAU - Sundberg, B
AU  - Sundberg B
FAU - Uzunel, M
AU  - Uzunel M
FAU - Ringden, O
AU  - Ringden O
FAU - Le Blanc, K
AU  - Le Blanc K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Cell Differentiation
MH  - Child
MH  - Choristoma
MH  - Female
MH  - Graft Survival
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Sheep
MH  - Young Adult
EDAT- 2012/05/04 06:00
MHDA- 2012/10/20 06:00
CRDT- 2012/05/04 06:00
PHST- 2012/05/04 06:00 [entrez]
PHST- 2012/05/04 06:00 [pubmed]
PHST- 2012/10/20 06:00 [medline]
AID - 10.1002/stem.1118 [doi]
PST - ppublish
SO  - Stem Cells. 2012 Jul;30(7):1575-8. doi: 10.1002/stem.1118.