PMID- 22553631
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20120906
LR  - 20211021
IS  - 2227-4898 (Electronic)
IS  - 2222-3959 (Print)
IS  - 2222-3959 (Linking)
VI  - 4
IP  - 2
DP  - 2011
TI  - Topical dihydroartemisinin inhibits suture-induced neovascularization in rat 
      corneas through ERK1/2 and p38 pathways.
PG  - 150-5
LID - 10.3980/j.issn.2222-3959.2011.02.08 [doi]
AB  - AIM: To determine if topical instillation of dihydroartemisinin (DHA) inhibits 
      corneal neovascularization (NV) in rats and to investigate the role of the 
      extracellular regulated kinases (ERK) 1/2 and p38 pathways in this process. 
      METHODS: Suture-induced corneal NV was produced in rats and the eyes were 
      topically treated with different concentrations of DHA (20mg/L, 10mg/L or 5mg/L) 
      or normal saline 4 times a day for 7 days. The corneal NV was quantified as the 
      proportion of NV area to the whole cornea. Western blot was used to determine the 
      expressions of vascular endothelial growth factor (VEGF) and the phosphorylation 
      status of VEGF receptor-2, ERK1/2 and p38 in the corneas. Immunofluorescent 
      staining was used to determine the expressions of phospho-ERK1/2 and phospho-p38 
      in the corneal tissues from the eyes treated with 20 mg/L DHA (DHA group) or 
      normal saline (control group). RESULTS: The proportion of corneal NV area in the 
      eyes treated with normal saline or DHA at dosages of 20mg/L, 10mg/L or 5mg/L was 
      (23.74+/-3.00)%, (15.73+/-2.88)%, (19.53+/-2.42)%, and (23.38+/-2.79)%, respectively. In 
      the eyes treated with 20mg/L or 10mg/L DHA, the corneal NV area was significantly 
      reduced when compared to that in eyes with normal saline (P<0.05). Western blot 
      analyses revealed that 20mg/L DHA significantly inhibited the expressions of VEGF 
      and phospho-VEGFR-2. Both 20mg/L and 10mg/L DHA inhibited the expressions of 
      phospho-ERK1/2 and phospho-p38. Immunofluorescent staining further demonstrated 
      that 20mg/L DHA lowered the expression levels of phospho-ERK1/2 and phospho-p38 
      in the corneas with suture-induced NV. CONCLUSION: Suture-induced NV in rat 
      corneas was significantly inhibited by topical treatment with 20mg/L and 10mg/L 
      DHA. The results suggest that the effects could be partially dependent on the 
      DHA-mediated inhibitions of the ERK1/2 and p38 pathways.
FAU - Zhong, Yan-Yan
AU  - Zhong YY
AD  - Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, 
      Guangzhou 510282, Guangdong Province, China.
FAU - Zhang, Hai-Feng
AU  - Zhang HF
FAU - Zhong, Jia-Xi
AU  - Zhong JX
FAU - Bai, Lang
AU  - Bai L
FAU - Lu, Xiao-He
AU  - Lu XH
LA  - eng
PT  - Journal Article
DEP - 20110418
PL  - China
TA  - Int J Ophthalmol
JT  - International journal of ophthalmology
JID - 101553860
PMC - PMC3340693
OTO - NOTNLM
OT  - cornea
OT  - dihydroartemisinin
OT  - extracellular regulated kinases 1/2
OT  - neovascularization
OT  - p38
EDAT- 2011/01/01 00:00
MHDA- 2011/01/01 00:01
PMCR- 2011/04/18
CRDT- 2012/05/04 06:00
PHST- 2011/03/18 00:00 [received]
PHST- 2011/04/02 00:00 [accepted]
PHST- 2012/05/04 06:00 [entrez]
PHST- 2011/01/01 00:00 [pubmed]
PHST- 2011/01/01 00:01 [medline]
PHST- 2011/04/18 00:00 [pmc-release]
AID - ijo-04-02-150 [pii]
AID - 10.3980/j.issn.2222-3959.2011.02.08 [doi]
PST - ppublish
SO  - Int J Ophthalmol. 2011;4(2):150-5. doi: 10.3980/j.issn.2222-3959.2011.02.08. Epub 
      2011 Apr 18.