PMID- 22553959
OWN - NLM
STAT- MEDLINE
DCOM- 20120910
LR  - 20181201
IS  - 1029-2330 (Electronic)
IS  - 1026-7158 (Linking)
VI  - 20
IP  - 5
DP  - 2012 Jun
TI  - Vaccine potential of cytosolic proteins loaded fibrin microspheres of 
      Cryptococcus neoformans in BALB/c mice.
PG  - 453-66
LID - 10.3109/1061186X.2012.685474 [doi]
AB  - Cryptococcosis is a leading mycological cause of mortality among immunologically 
      compromised individuals. In order to develop an effective vaccine against 
      Cryptococcus neoformans, the cytosolic proteins (Cp) of the pathogen have been 
      used as an antigen in combination with different formulations. In the present 
      study, we have demonstrated that Cp encapsulated poly-lactide co-glycolide (PLGA) 
      microsphere further co-encapsulated into the biocompatible fibrin cross-linked 
      plasma beads (Fib-PLGA-Cp) mediated cytosolic delivery elicited strong immune 
      response in the BALB/c mice. In contrast, other formulations of Cp failed to 
      impart significant level of protection. The immune response, involved with 
      Fib-PLGA-Cp protection, appear to interact with the target cells by both 
      endocytosis as well as membrane fusion mode, thus helping in the activation of 
      both CD4(+) and CD8(+) T-cells. Analysis of cytokine profiles in immunized 
      animals revealed that the protective response was associated with the Th1/Th2 
      polarization in favor of type-1 cytokine [interferons (IFN)-gamma and interleukin 
      (IL)-2] cells. Furthermore, vaccination with Fib-PLGA-Cp elicited high 
      immunoglobulin (Ig) G(l) and IgG(2a) isotype response; successfully cleared 
      fungal burden in vital organs and also increased the survival rate of immunized 
      animals. Altogether the present study is a clear indicative of the possible use 
      of fibrin microsphere-based targeted delivery of cytosolic proteins to induce 
      protective immune responses against experimental murine cryptococcosis.
FAU - Khan, Azmat Ali
AU  - Khan AA
AD  - Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India. 
      azmatbiotech@gmail.com
FAU - Jabeen, Mumtaz
AU  - Jabeen M
FAU - Chauhan, Arun
AU  - Chauhan A
FAU - Owais, Mohammad
AU  - Owais M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120504
PL  - England
TA  - J Drug Target
JT  - Journal of drug targeting
JID - 9312476
RN  - 0 (Antigens, Fungal)
RN  - 0 (Cytokines)
RN  - 0 (Fungal Proteins)
RN  - 0 (Fungal Vaccines)
RN  - 0 (Immunoglobulin G)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9001-31-4 (Fibrin)
SB  - IM
MH  - Animals
MH  - Antigens, Fungal/immunology
MH  - Cryptococcosis/immunology/*prevention & control
MH  - Cryptococcus neoformans/*isolation & purification
MH  - Cytokines/immunology
MH  - Cytosol/metabolism
MH  - Disease Models, Animal
MH  - Endocytosis/immunology
MH  - Female
MH  - Fibrin/chemistry
MH  - Fungal Proteins/*immunology
MH  - Fungal Vaccines/*immunology
MH  - Immunoglobulin G/immunology
MH  - Lactic Acid/chemistry
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microspheres
MH  - Polyglycolic Acid/chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Survival Rate
EDAT- 2012/05/05 06:00
MHDA- 2012/09/11 06:00
CRDT- 2012/05/05 06:00
PHST- 2012/05/05 06:00 [entrez]
PHST- 2012/05/05 06:00 [pubmed]
PHST- 2012/09/11 06:00 [medline]
AID - 10.3109/1061186X.2012.685474 [doi]
PST - ppublish
SO  - J Drug Target. 2012 Jun;20(5):453-66. doi: 10.3109/1061186X.2012.685474. Epub 
      2012 May 4.