PMID- 22555532
OWN - NLM
STAT- MEDLINE
DCOM- 20120726
LR  - 20220408
IS  - 0001-5547 (Print)
IS  - 0001-5547 (Linking)
VI  - 56
IP  - 3
DP  - 2012
TI  - Comparison of breast carcinoma prognostic/predictive biomarkers on cell blocks 
      obtained by various methods: Cellient, formalin and thrombin.
PG  - 289-96
LID - 10.1159/000337436 [doi]
AB  - OBJECTIVE: To compare results of immunohistochemical (IHC) assays for estrogen 
      receptor (ER), progesterone receptor (PR) and human epidermal growth factor 
      receptor 2 (HER2) performed on thrombin, formalin and Cellient cell blocks to 
      those performed on tissue. STUDY DESIGN: Formalin, thrombin and Cellient cell 
      blocks were prepared from cytologic samples obtained from resection specimens of 
      31 patients with invasive breast carcinoma. ER, PR, HER2 and MIB-1 (Ki-67) IHC 
      stains were performed on all three types of cell blocks and compared to the same 
      stains performed on the patient's paraffin-embedded biopsy or resection. Cell and 
      tissue blocks with equivocal staining for HER2 were submitted for fluorescence in 
      situ hybridization (FISH). RESULTS: Adequate Cellient blocks were obtained for 
      all 31 cases. Comparison of results of ER IHC assays on all three types of cell 
      blocks showed 100% correlation with tissue. Both Cellient and thrombin blocks 
      showed 100% correlation with tissue for HER2 IHC and FISH results. The only 
      statistically significant difference between cell block methods was found in PR 
      staining, where false-negative results occurred with Cellient and thrombin 
      blocks. CONCLUSION: Breast biomarker IHC assays performed on Cellient blocks are 
      reliable and correlate with tissue block results, particularly for ER and HER2, 
      the most clinically important markers.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Gorman, Blythe K
AU  - Gorman BK
AD  - Department of Pathology and Genomic Medicine, The Methodist Hospital, Houston, TX 
      77030, USA. bkgorman@tmhs.org
FAU - Kosarac, Ognjen
AU  - Kosarac O
FAU - Chakraborty, Subhendu
AU  - Chakraborty S
FAU - Schwartz, Mary R
AU  - Schwartz MR
FAU - Mody, Dina R
AU  - Mody DR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20120426
PL  - Switzerland
TA  - Acta Cytol
JT  - Acta cytologica
JID - 0370307
RN  - 0 (Biomarkers, Tumor)
RN  - 1HG84L3525 (Formaldehyde)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*analysis
MH  - Biopsy, Fine-Needle/*methods
MH  - Breast Neoplasms/*pathology
MH  - Cytodiagnosis/*methods
MH  - Epithelial Cells/*pathology
MH  - Female
MH  - Formaldehyde
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Invasiveness/*pathology
MH  - Thrombin
EDAT- 2012/05/05 06:00
MHDA- 2012/07/27 06:00
CRDT- 2012/05/05 06:00
PHST- 2012/01/10 00:00 [received]
PHST- 2012/02/09 00:00 [accepted]
PHST- 2012/05/05 06:00 [entrez]
PHST- 2012/05/05 06:00 [pubmed]
PHST- 2012/07/27 06:00 [medline]
AID - 000337436 [pii]
AID - 10.1159/000337436 [doi]
PST - ppublish
SO  - Acta Cytol. 2012;56(3):289-96. doi: 10.1159/000337436. Epub 2012 Apr 26.