PMID- 22558105
OWN - NLM
STAT- MEDLINE
DCOM- 20120910
LR  - 20240315
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 4
DP  - 2012
TI  - Ss-Sl2, a novel cell wall protein with PAN modules, is essential for sclerotial 
      development and cellular integrity of Sclerotinia sclerotiorum.
PG  - e34962
LID - 10.1371/journal.pone.0034962 [doi]
LID - e34962
AB  - The sclerotium is an important dormant body for many plant fungal pathogens. 
      Here, we reported that a protein, named Ss-Sl2, is involved in sclerotial 
      development of Sclerotinia sclerotiorum. Ss-Sl2 does not show significant 
      homology with any protein of known function. Ss-Sl2 contains two putative PAN 
      modules which were found in other proteins with diverse adhesion functions. 
      Ss-Sl2 is a secreted protein, during the initial stage of sclerotial development, 
      copious amounts of Ss-Sl2 are secreted and accumulated on the cell walls. The 
      ability to maintain the cellular integrity of RNAi-mediated Ss-Sl2 silenced 
      strains was reduced, but the hyphal growth and virulence of Ss-Sl2 silenced 
      strains were not significantly different from the wild strain. Ss-Sl2 silenced 
      strains could form interwoven hyphal masses at the initial stage of sclerotial 
      development, but the interwoven hyphae could not consolidate and melanize. Hyphae 
      in these interwoven bodies were thin-walled, and arranged loosely. 
      Co-immunoprecipitation and yeast two-hybrid experiments showed that 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Woronin body major protein 
      (Hex1) and elongation factor 1-alpha interact with Ss-Sl2. GAPDH-knockdown 
      strains showed a similar phenotype in sclerotial development as Ss-Sl2 silenced 
      strains. Hex1-knockdown strains showed similar impairment in maintenance of 
      hyphal integrity as Ss-Sl2 silenced strains. The results suggested that Ss-Sl2 
      functions in both sclerotial development and cellular integrity of S. 
      sclerotiorum.
FAU - Yu, Yang
AU  - Yu Y
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan, Hubei Province, People's Republic of China.
FAU - Jiang, Daohong
AU  - Jiang D
FAU - Xie, Jiatao
AU  - Xie J
FAU - Cheng, Jiasen
AU  - Cheng J
FAU - Li, Guoqing
AU  - Li G
FAU - Yi, Xianhong
AU  - Yi X
FAU - Fu, Yanping
AU  - Fu Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120425
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Fungal Proteins)
RN  - 0 (Melanins)
RN  - 0 (Peptide Elongation Factor 1)
RN  - 79956-01-7 (Polyketide Synthases)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Ascomycota/*cytology/growth & development/pathogenicity
MH  - Base Sequence
MH  - Cell Wall/*chemistry
MH  - Chromatography, Liquid
MH  - Fungal Proteins/*genetics
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism
MH  - Hyphae/*metabolism
MH  - Immunoprecipitation
MH  - Melanins/biosynthesis
MH  - Molecular Sequence Data
MH  - Mycelium/growth & development/*ultrastructure
MH  - Peptide Elongation Factor 1/metabolism
MH  - Polyketide Synthases/genetics
MH  - RNA Interference
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Tandem Mass Spectrometry
MH  - Two-Hybrid System Techniques
MH  - Virulence
PMC - PMC3338822
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/05/05 06:00
MHDA- 2012/09/11 06:00
PMCR- 2012/04/25
CRDT- 2012/05/05 06:00
PHST- 2011/11/24 00:00 [received]
PHST- 2012/03/08 00:00 [accepted]
PHST- 2012/05/05 06:00 [entrez]
PHST- 2012/05/05 06:00 [pubmed]
PHST- 2012/09/11 06:00 [medline]
PHST- 2012/04/25 00:00 [pmc-release]
AID - PONE-D-11-24640 [pii]
AID - 10.1371/journal.pone.0034962 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(4):e34962. doi: 10.1371/journal.pone.0034962. Epub 2012 Apr 25.