PMID- 22559671
OWN - NLM
STAT- MEDLINE
DCOM- 20120831
LR  - 20211021
IS  - 1560-2281 (Electronic)
IS  - 1083-3668 (Print)
IS  - 1083-3668 (Linking)
VI  - 17
IP  - 4
DP  - 2012 Apr
TI  - In vivo imaging of unstained tissues using a compact and flexible multiphoton 
      microendoscope.
PG  - 040505
LID - 10.1117/1.JBO.17.4.040505 [doi]
LID - 040505
AB  - We use a compact and flexible multiphoton microendoscope (MPME) to acquire in 
      vivo images of unstained liver, kidney, and colon from an anesthetized rat. The 
      device delivers femtosecond pulsed 800 nm light from the core of a raster-scanned 
      dual-clad fiber (DCF), which is focused by a miniaturized gradient-index lens 
      assembly into tissue. Intrinsic fluorescence and second-harmonic generation 
      signal from the tissue is epi-collected through the core and inner clad of the 
      same DCF. The MPME has a rigid distal tip of 3 mm in outer diameter and 4 cm in 
      length. The image field-of-view measures 115 mum by 115 mum and was acquired at 4.1 
      frames/s with 75 mW illumination power at the sample. Organs were imaged after 
      anesthetizing Sprague-Dawley rats with isofluorane gas, accessing tissues via a 
      ventral-midline abdominal incision, and isolating the organs with tongue 
      depressors. In vivo multiphoton images acquired from liver, kidney, and colon 
      using this device show features similar to that of conventional histology slides, 
      without motion artifact, in ~75% of imaged frames. To the best of our knowledge, 
      this is the first demonstration of multiphoton imaging of unstained tissue from a 
      live subject using a compact and flexible MPME device.
FAU - Brown, Christopher M
AU  - Brown CM
FAU - Rivera, David R
AU  - Rivera DR
FAU - Pavlova, Ina
AU  - Pavlova I
FAU - Ouzounov, Dimitre G
AU  - Ouzounov DG
FAU - Williams, Wendy O
AU  - Williams WO
FAU - Mohanan, Sunish
AU  - Mohanan S
FAU - Webb, Watt W
AU  - Webb WW
FAU - Xu, Chris
AU  - Xu C
LA  - eng
GR  - R01 CA133148/CA/NCI NIH HHS/United States
GR  - R01 EB006736/EB/NIBIB NIH HHS/United States
GR  - R01-CA133148/CA/NCI NIH HHS/United States
GR  - R01-EB006736/EB/NIBIB NIH HHS/United States
PT  - Letter
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Biomed Opt
JT  - Journal of biomedical optics
JID - 9605853
SB  - IM
MH  - Animals
MH  - Colon/chemistry
MH  - *Endoscopes
MH  - Fiber Optic Technology/instrumentation
MH  - Kidney/chemistry
MH  - Liver/chemistry
MH  - Male
MH  - Microscopy, Fluorescence, Multiphoton/*instrumentation/*methods
MH  - Microtechnology/*instrumentation
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC3382343
EDAT- 2012/05/09 06:00
MHDA- 2012/09/01 06:00
PMCR- 2013/04/09
CRDT- 2012/05/08 06:00
PHST- 2012/05/08 06:00 [entrez]
PHST- 2012/05/09 06:00 [pubmed]
PHST- 2012/09/01 06:00 [medline]
PHST- 2013/04/09 00:00 [pmc-release]
AID - 12043L [pii]
AID - 10.1117/1.JBO.17.4.040505 [doi]
PST - ppublish
SO  - J Biomed Opt. 2012 Apr;17(4):040505. doi: 10.1117/1.JBO.17.4.040505.